Research Catalog

T cell protocols

Title
T cell protocols / edited by Gennaro De Libero.
Publication
Totowa, N.J. : Humana Press, 2009.

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TextRequest in advance QR185.8.T2 T14 2009gOff-site

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Details

Additional Authors
De Libero, Gennaro.
Description
xi, 165 pages, 3 pages of plates : illustrations (some color); 25 cm
Summary
"With a wide variety of investigative approaches, T cell immunology is a vital and open field of study. In T Cell Protocols, Second Edition, an international panel of experts contribute fully updated classic protocols as well as newly established novel techniques for the study of T lymphocyte biology. The chapters in this volume provide brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections which collect expert tips on troubleshooting and avoiding known pitfalls."--BOOK JACKET.
Subjects
Contents
Color Plates -- 1. Analysis of Frequency and Phenotype of Antigen-Specific T Cells / Angus Stock and Vincenzo Cerundolo -- 2. B Cell Helper Assays / Sergio Abrignani, Elena Tonti, Giulia Casorati and Paolo Dellabona -- 3. Transkingdom RNA Interference (tkRNAi): A Novel Method to Induce Therapeutic Gene Silencing / Thu A. Nguyen and Johannes H. Fruehauf -- 4. Flow Cytometry and Cell Activation / Sonia Gavasso -- 5. Investigating T Cells by Polychromatic Flow Cytometry / Enrico Lugli, Leonarda Troiano and Andrea Cossarizza -- 6. Generation of Human T Cell Clones / Sabrina Mariotti and Roberto Nisini -- 7. Limiting Dilution Analysis of Antigen-Specific T Cells / Jorge Carneiro, Lurdes Duarte and Elisabetta Padovan -- 8. T Cell Epitope-Mapping by Cytokine Gene Expression Assay / Maurizio Provenzano and Giulio C. Spagnoli -- 9. Cytokine Multiplex Immunoassay: Methodology and (Clinical) Applications / Wilco de Jager, Berent Prakken and Ger T. Rijkers -- 10. Purification of the T Cell Antigen Receptor and Analysis by Blue-Native PAGE / Mahima Swamy and Wolfgang W. A. Schamel -- 11. Non-Replicating Recombinant Vaccinia Virus Expressing CD80 to Enhance T-Cell Stimulation / Paul Zajac.
ISBN
  • 9781588295873 (print)
  • 1588295877 (print)
  • 9781603275279 (e-ISBN)
  • 1603275274 (e-ISBN)
OCLC
  • ocn227033896
  • 227033896
  • SCSB-5446829
Owning Institutions
Columbia University Libraries