Research Catalog
Plant hormones : methods and protocols
- Title
- Plant hormones : methods and protocols / edited by Sean Cutler and Dario Bonetta.
- Publication
- Totowa, N.J. : Humana ; London : Springer [distributor], 2009.
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2 Items
| Status | Format | Access | Call Number | Item Location |
|---|---|---|---|---|
| Text | Request in advance | QH506 .M56 v.495 | Off-site | |
Not available - Please for assistance. | Text | Use in library | QK898.H67 P53 2009g | Off-site |
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Details
- Additional Authors
- Description
- x, 146 pages : illustrations; 27 cm.
- Summary
- "In Plant Hormones: Methods and Protocols, Second Edition, expert researchers explore the most current genetic, biochemical, analytical and chemical biological approaches for understanding plant hormone action, providing a concise overview of methods and reagents needed to dissect plant signalling pathways using chemical genetic methods. Chapters address such topics as genetic methods of analysis using the model system Arabidopsis thaliana, biochemical methods for documenting interactions between hormones and receptors, the use of high-throughput sequencing technologies for microRNA analysis, and analytical approaches for measuring endogenous plant hormone levels, including LC-MS and GC-MS based methods. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls."--BOOK JACKET.
- Series Statement
- Methods in molecular biology ; 495
- Uniform Title
- Methods in molecular biology ; v. 495.
- Subjects
- Genre/Form
- Laboratory Manual.
- Bibliography (note)
- Includes bibliographical references and index.
- Contents
- 1. Using Reverse Genetics to Develop Small Knockout Collections for Specific Biological Questions / Julian Northey and Peter McCourt -- 2. Visualizing Auxin Transport Routes in Arabidopsis Leaf Primordia / Danielle Marcos and Thomas Berleth -- 3. Gene Expression Analyses for Elucidating Mechanisms of Hormonal Action in Plants / George W. Bassel and Nicholas J. Provart -- 4. Measurement of Plant Hormones by Liquid Chromatography-Mass Spectrometry / Stacey J. Owen and Suzanne R. Abrams -- 5. Measurement of Abscisic Acid and Gibberellins by Gas Chromatography/Mass Spectrometry / Masanori Okamoto, Atsushi Hanada, Yuji Kamiya, Shinjiro Yamaguchi and Eiji Nambara -- 6. Pull-Down Assays for Plant Hormone Research / Stefan Kepinski -- 7. Binding Assays for Brassinosteroid Receptors / Ana Cano-Delgado and Zhi-Yong Wang -- 8. Binding Assays for Abscisic Acid Receptors / Fawzi A. Razem and Robert D. Hill -- 9. Cytokinin Sensing Systems Using Microorganisms / Masayuki Higuchi, Tatsuo Kakimoto and Takeshi Mizuno -- 10. Hormone-Binding Assay Using Living Bacteria Expressing Eukaryotic Receptors / Georgy A. Romanov and Sergey N. Lomin -- 11. Discovery of Plant MicroRNAs and Short-Interfering RNAs by Deep Parallel Sequencing / Padmanabhan Chellappan and Hailing Jin -- 12. The Use of Chemical Genomics to Investigate Pathways Intersecting Auxin-Dependent Responses and Endomembrane Trafficking in Arabidopsis Thaliana / Lorena Norambuena, Glenn R. Hicks and Natasha V. Raikhel.
- ISBN
- 9781934115329 (hbk.)
- 1934115320 (hbk.)
- OCLC
- ocn237196786
- 237196786
- SCSB-5448552
- Owning Institutions
- Columbia University Libraries