Research Catalog

Title

HIV protocols / edited by Vinayaka R. Prasad and Ganjam V. Kalpana.

Publication

New York, NY : Humana Press, [2009], ©2009.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Prasad, Vinayaka R.
• Kalpana, Ganjam V.

Description

xiv, 457 pages : illustrations; 27 cm.

Series Statement

• Methods in molecular biology ; 485
• Springer protocols

Uniform Title

• Methods in molecular biology (Clifton, N.J.) ; v. 485.
• Springer protocols (Series)

Subjects

• Immunologic Techniques
• HIV (Viruses) > Laboratory manuals
• HIV infections > Laboratory manuals
• HIV-1
• Virology > methods
• Laboratory Manual
• AIDS (Disease) > Laboratory manuals
• Clinical Protocols
• HIV Infections
• Molecular Biology > methods

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

Sect. I. Preparation of Virus Particles and Their Analysis -- 1. Methods for Viral RNA Isolation and PCR Amplification for Sequencing of Near Full-Length HIV-1 Genomes / Kimdar Sherefa Kemal, Milan Reinis, Barbara Weiser and Harold Burger -- 2. Purification of HIV-1 Virions by Subtilisin Digestion or CD45 Immunoaffinity Depletion for Biochemical Studies / David E. Ott -- 3. Calculating HIV-1 Infectious Titre Using a Virtual TCID[subscript 50] Method / Yong Gao, Immaculate Nankya, Awet Abraha, Ryan M. Troyer, Kenneth N. Nelson, Andrea Rubio and Eric J. Arts -- Sect. II. Methods to Study HIV-1 Replication -- 4. Cell-Free Assays for HIV-1 Uncoating / Christopher Aiken -- 5. Real-Time PCR Analysis of HIV-1 Replication Postentry Events / Jean L. Mbisa, Krista A. Delviks-Frankenberry, James A. Thomas, Robert J. Gorelick and Vinay K. Pathak -- 6. Analysis of 2-LTR Circle Junctions of Viral DNA in Infected Cells / Dibyakanti Mandal and Vinayaka R. Prasad -- 7. HIV-1 Recombination: An Experimental Assay and a Phylogenetic Approach / Michael D. Moore, Mario P. S. Chin and Wei-Shau Hu -- 8. Methods of Preparation and Analysis of Intracellular Reverse Transcription Complexes / Ariberto Fassati -- 9. Analysis of Viral and Cellular Proteins in HIV-1 Reverse Transcription Complexes by Co-immunoprecipitation / Sergey N. Iordanskiy and Michael I. Bukrinsky -- 10. Isolation and Analysis of HIV-1 Preintegration Complexes / Alan Engelman -- 11. Bisarsenical Labeling of HIV-1 for Real-Time Fluorescence Microscopy / Nathalie J. Arhel and Pierre Charneau -- 12. Methods for the Study of HIV-1 Assembly / Abdul A. Waheed, Akira Ono and Eric O. Freed -- 13. Assembly of Immature HIV-1 Capsids Using a Cell-Free System / Jaisri R. Lingappa and Beth K. Thielen -- 14. Preparation of Recombinant Hiv-1 Gag Protein and Assembly of Virus-Like Particles In Vitro / Siddhartha A. K. Datta and Alan Rein -- 15. Methods for the Analysis of HIV-1 Nucleocapsid Protein Interactions with Oligonucleotides / Andrew G. Stephen and Robert J. Fisher -- 16. Methods for Analysis of Incorporation and Annealing of tRNA[superscript Lys] in HIV-1 / Shan Cen, Fei Guo and Lawrence Kleiman -- Sect. III. Specialized Approaches to Study HIV-1 Biology and Pathogenesis -- 17. Somatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors / Susana T. Valente and Stephen P. Goff -- 18. Rapid, Controlled and Intensive Lentiviral Vector-Based RNAi / Manuel Llano, Natassia Gaznick and Eric M. Poeschla -- 19. Reverse Two-Hybrid Screening to Analyze Protein-Protein Interaction of HIV-1 Viral and Cellular Proteins / Supratik Das and Ganjam V. Kalpana -- 20. Methods to Study Monocyte Migration Induced by HIV-infected Cells / Vasudev R. Rao, Eliseo A. Eugenin, Joan W. Berman and Vinayaka R. Prasad -- 21. Novel Mouse Models for Understanding HIV-1 Pathogenesis / Aviva Joseph, Kaori Sango and Harris Goldstein -- Sect. IV. Immunological Studies of HIV -- 22. Mucosal Antibody Responses to HIV / Zina Moldoveanu and Jiri Mestecky -- 23. Isolating Mucosal Lymphocytes from Biopsy Tissue for Cellular Immunology Assays / Barbara L. Shacklett, J. William Critchfield and Donna Lemongello -- 24. Quantifying HIV-1-Specific CD8+ T-Cell Responses Using ELISPOT and Cytokine Flow Cytometry / Barbara L. Shacklett, J. William Critchfield and Donna Lemongello -- 25. Multiparameter Flow Cytometry Monitoring of T Cell Responses / Holden T. Maecker -- 26. Measuring HIV Neutralization in a Luciferase Reporter Gene Assay / David C. Montefiori -- 27. Assessing the Antiviral Activity of HIV-1 -Specific Cytotoxic T Lymphocytes / Otto O. Yang -- 28. Methods for Quantitating Antigen-Specific T Cell Responses Using Functional Assays in Rhesus Macaques / Rama Rao Amara -- Sect. V. Drug Resistant Viruses and Viral Evolution -- 29. Isolation of Drug-Resistant Mutant HIV Variants Using Tissue Culture Drug Selection / Maureen Oliveira, Bluma G. Brenner and Mark A. Wainberg -- 30. Virus Evolution as a Tool to Study HIV-1 Biology / Ben Berkhout and Atze T. Das.

ISBN

• 9781588298591 (alk. paper)
• 1588298590 (alk. paper)

LCCN

2008938341

OCLC

• ocn244765671
• 244765671
• SCSB-5449636

Owning Institutions

Columbia University Libraries