Research Catalog

Title

Candida albicans : methods and protocols / edited by Ronald L. Cihlar and Richard A. Calderone.

Publication

Totowa, N.J. : Humana Press, [2009], ©2009.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Cihlar, Ronald L.
• Calderone, Richard A., 1942-

Description

x, 206 pages : illustrations; 27 cm.

Series Statement

Methods in molecular biology ; 499

Uniform Title

Methods in molecular biology (Clifton, N.J.) ; v. 499.

Subject

• Candida albicans
• Candidiasis
• Pathogenic fungi

Bibliography (note)

• Includes bibliographical references and index.

Contents

Pt. I. Immunogical methods -- 1. Isolation of dendritic cells from human blood for in vitro interaction studies with fungal antigens / T. Sreevalsan -- 2. Detection and quantitiaton of anti-fungal SIgA antibodies in body fluids / Michael F. Cole -- 3. Phagocytosis and killing assays for Candida species / Chen Du and Richard Calderone -- 4. Immunization protocols for use in animal models of candidiasis / Esther Segal and Hana Sandovsky-Losica -- Pt. II. Virulence and biofilms -- 5. Penetration of antifungal agents through Candida biofilms / L. Julia Douglas -- 6. Candidal biofilm analysis in the artificial throat using FISH / Bastiaan P. Krom, Kevin Buijssen, Henk J. Bussceher and Henny C. van der Mei -- 7. Conditions for optimal Candida biofilm development in microtiter plates / Bastiaan P. Krom, Jesse B. Cohen, Gail McElhaney-Feser, Henk J. Busscher, Henny C. van der Mei and Ronald L. Cihlar -- Pt. III. Virulence measurements: In vitro, ex vivo, and in vivo -- 8. Animal models of candidiasis / Cornelius J. Clancy, Shaoji Cheng and Minh Hong Nguyen -- 9. Candida albicans gene expression in an in vivo infection model / Michael D. Kruppa -- 10. In vitro and ex vivo assays of virulence in Candida albicans / Richard A. Calderone -- Pt. IV. Strain typing and identification -- 11. Biotyping of Candida albicans and other fungi by yeast killer toxins sensitivity / Luciano Polonelli and Stefania Conti -- 12. DNA fingerprinting Candida species / Claude Pujol and David R. Soll -- Pt. V. Genomics and Proteomics -- 13. The application of tandem-affinity purification to Candida albicans / Chris Blackwell and Jeremy D. Brown -- 14. Preparation of samples for proteomic analysis of the Candida albicans cell wall / Neeraj Chauhan -- 15. Reporter Gene assays in Candida albicans / Joy Sturtevant -- 16. Genetic Transformation of Candida albicans / Ana M. Ramon and William A. Fonzi -- 17. Large-scale gene disruption using the UAU1 Cassette / Clarissa J. Nobile and Aaron P. Mitchell -- Pt. VI. Appendix -- 18. Standard growth media and common techniques for use with Candida albicans / Neeraj Chauhan and Michael D. Kruppa.

ISBN

• 1588297608
• 9781588297600

LCCN

2008942152

OCLC

• ocn262510959
• 262510959
• SCSB-5455444

Owning Institutions

Columbia University Libraries