Research Catalog

Title

Phospho-proteomics : methods and protocols / edited by Marjo de Graauw.

Publication

Totowa, N.J. : Humana ; London : Springer [distributor], 2009.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Graauw, Marjo de, 1978-

Description

xi, 328 pages : illustrations (some color); 27 cm.

Series Statement

Methods in molecular biology ; 527

Uniform Title

Methods in molecular biology ; v. 527.

Subjects

• Proteomics
• Phosphorylation
• Proteomics > methods
• Protein kinases
• Phosphoproteins

Bibliography (note)

• Includes bibliographical references and index.

Contents

Pt. I. Protein Phosphorylation and 2D Gel Electrophoresis -- 1. A High-Resolution Two Dimensional Gel- and Pro-Q DPS-Based Proteomics Workflow for Phosphoprotein Identification and Quantitative Profiling / Ganesh K. Agrawal and Jay J. Thelen -- 2. Differential Phosphoprotein Labelling (DIPPL) Using [superscript 32]P and [superscript 33]P / Aviva M. Tolkovsky and Andreas Wyttenbach -- Pt. II. Enrichment of Phosphoproteins and Peptides -- 3. Identification of Oxidative Stress-Induced Tyrosine Phosphorylated Proteins by Immunoprecipitation and Mass Spectrometry / M. Carmen Duran, Hong-Lin Chan and John F. Timms -- 4. Enrichment and Characterization of Phosphopeptides by Immobilized Metal Affinity Chromatography (IMAC) and Mass Spectrometry / Tine E. Thingholm and Ole N. Jensen -- 5. The Use of Titanium Dioxide Micro-Columns to Selectively Isolate Phosphopeptides from Proteolytic Digests / Tine E. Thingholm and Martin R. Larsen -- 6. Enrichment and Separation of Mono- and Multiply Phosphorylated Peptides Using Sequential Elution from IMAC Prior to Mass Spectrometric Analysis / Tine E. Thingholm, Ole N. Jensen and Martin R. Larsen -- 7. Use of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Phosphotyrosine Protein Identification and Quantitation / Guoan Zhang and Thomas A. Neubert -- 8. Hydrophilic Interaction Chromatography for Fractionation and Enrichment of the Phosphoproteome / Dean E. McNulty and Roland S. Annan -- 9. SILAC for Global Phosphoproteomic Analysis / Genaro Pimienta, Raghothama Chaerkady and Akhilesh Pandey -- 10. Quantitative Phospho-proteomics Based on Soluble Nanopolymers / Anton Iliuk and W. Andy Tao -- 11. Profiling the Tyrosine Phosphorylation State Using SH2 Domains / Kevin Dierck, Kazuya Machida, Bruce J. Mayer and Peter Nollau -- Pt. III. Phosphoprotein Analysis by Mass Spectrometry -- 12. An Overview of the Qualitative Analysis of Phosphoproteins by Mass Spectrometry / Philip R. Gafken -- 13. The Analysis of Phosphoproteomes by Selective Labelling and Advanced Mass Spectrometric Techniques / Angela Amoresano, Claudia Cirulli, Gianluca Monti, Eric Quemeneur and Gennaro Marino -- 14. On-Line Liquid Chromatography Electron Capture Dissociation for the Characterization of Phosphorylation Sites in Proteins / Steve M. M. Sweet and Helen J. Cooper -- 15. Quantification of Protein Phosphorylation by [actual symbol not reproducible]LC-ICP-MS / Ralf Kruger, Nico Zinn and Wolf D. Lehmann -- 16. Reverse-Phase Diagonal Chromatography for Phosphoproteome Research / Kris Gevaert and Joel Vandekerckhove -- 17. Chemical Tagging Strategies for Mass Spectrometry-Based Phospho-proteomics / Alexander Leitner and Wolfgang Lindner -- Pt. IV. Arrays to Study Protein-Phosphorylation -- 18. Antibody Array Platform to Monitor Protein Tyrosine Phosphorylation in Mammalian Cells / Alicia S. Chung and T. Eugene Chin -- 19. Protein Tyrosine Kinase Characterization Based on Fully Automated Synthesis of (Phospho)Peptide Arrays in Microplates / W. Carl Saxinger -- 20. Kinome Profiling Using Peptide Arrays in Eukaryotic Cells / Kaushal Parikh, Maikel P. Peppelenbosch and Tita Ritsema -- Pt. V. Bioinformatics -- 21. ProMoST: A Tool for Calculating the p I and Molecular Mass of Phosphorylated and Modified Proteins on Two-Dimensional Gels / Brian D. Halligan -- 22. Kinase-Specific Prediction of Protein Phosphorylation Sites / Martin L. Miller and Nikolaj Blom -- 23. Reconstructing Regulatory Kinase Pathways from Phosphopeptide Data: A Bioinformatics Approach / Lawrence G. Puente, Robin E. C. Lee and Lynn A. Megeney.

ISBN

• 9781603278331 (hbk.)
• 1603278338 (hbk.)
• 9781603278348 (e-ISBN)
• 1603278346 (e-ISBN)

LCCN

2008944207

OCLC

• ocn263296009
• 263296009
• SCSB-5462023

Owning Institutions

Columbia University Libraries