Research Catalog

Title

Toll-like receptors : methods and protocols / edited by Claire E. McCoy and Luke A.J. O'Neill.

Publication

Totowa, N.J. : Humana ; London : Springer [distributor], 2009.

Items in the Library & Off-site

Details

Additional Authors

• McCoy, Claire E.
• O'Neill, Luke A. J.

Description

xiv, 447 pages : illustrations (some color); 27 cm.

Series Statement

• Methods in molecular biology, 1064-3745; 517
• Springer protocols

Uniform Title

• Methods in molecular biology.
• Springer protocols (Series)

Subjects

• Membrane Glycoproteins > immunology
• Cell receptors
• Immunity, Cellular
• Receptors, Cell Surface
• Cellular immunity

Bibliography (note)

• Includes bibliographical references and index.

Contents

Pt. I. Methods to Detect and Analyze Toll-Like Receptors -- 1. Expression Analysis of the Toll-Like Receptors in Human Peripheral Blood Mononuclear Cells / Jakub Siednienko and Sinead M. Miggin -- 2. Ligands, Cell-Based Models, and Readouts Required for Toll-Like Receptor Action / Jerome Dellacasagrande -- 3. Toll-Like Receptor Interactions Imaged by FRET Microscopy and GFP Fragment Reconstitution / Gabor Horvath, Scott Young and Eicke Latz -- 4. Predicting Toll-Like Receptor Structures and Characterizing Ligand Binding / Joshua N. Leonard, Jessica K. Bell and David M. Segal -- 5. Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures / Tom P. Monie, Nicholas J. Gay and Monique Gangloff -- 6. Expression, Purification, and Crystallization of Toll/Interleukin-1 Receptor (TIR) Domains / Xiao Tao and Liang Tong -- Pt. II. Methods to Analyze Signal Transduction Downstream of Toll-Like Receptor Stimulation -- 7. Proteomic Analysis of Protein Complexes in Toll-Like Receptor Biology / Kiva Brennan and Caroline A. Jefferies -- 8. 2D-DIGE: Comparative Proteomics of Cellular Signalling Pathways / Nadia Ben Larbi and Caroline Jefferies -- 9. MAPPIT (Mammalian Protein-Protein Interaction Trap) Analysis of Early Steps in Toll-Like Receptor Signalling / Peter Ulrichts, Irma Lemmens, Delphine Lavens, Rudi Beyaert and Jan Tavernier -- 10. Analysis of the Functional Role of Toll-Like Receptor-4 Tyrosine Phosphorylation / Andrei E. Medvedev and Wenji Piao -- 11. Analysis of Ubiquitin Degradation and Phosphorylation of Proteins / Pearl Gray -- 12. The Generation of Highly Purified Primary Human Neutrophils and Assessment of Apoptosis in Response to Toll-Like Receptor Ligands / Lisa C. Parker, Lynne R. Prince, David J. Buttle and Ian Sabroe -- 13. Cellular Expression of A20 and ABIN-3 in Response to Toll-Like Receptor-4 Stimulation / Kelly Verhelst, Lynn Verstrepen, Beatrice Coornaert, Isabelle Carpentier and Rudi Beyaert -- 14. Characterisation of Viral Proteins that Inhibit Toll-Like Receptor Signal Transduction / Julianne Stack and Andrew G. Bowie -- Pt. III. Genetic Techniques in Toll-Like Receptor Analysis -- 15. Genetic Dissection of Toll-Like Receptor Signaling Using ENU Mutagenesis / Kasper Hoebe -- 16. Microarray Experiments to Uncover Toll-Like Receptor Function / Harry Bjorkbacka -- 17. Uncovering Novel Gene Function in Toll-Like Receptor Signalling Using siRNA / Michael Carty, Sinead Keating and Andrew Bowie -- 18. Genotyping Methods to Analyse Polymorphismsin Toll-Like Receptors and Disease / Chiea-Chuen Khor -- Pt. IV. Tool-Like Receptors and Disease -- 19. Experimental Models of Acute Infection and Toll-Like Receptor Driven Septic Shock / Ruth Ferstl, Stephan Spiller, Sylvia Fichte, Stefan Dreher and Carsten J. Kirschning -- 20. Toll-Like Receptors and Rheumatoid Arthritis / Fabia Brentano, Diego Kyburz and Steffen Gay -- 21. Practical Techniques for Detection of Toll-Like Receptor-4 in the Human Intestine / Ryan Ungaro, Maria T. Abreu and Masayuki Fukata -- 22. Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells / Melissa B. Uccellini, Ana M. Avalos, Ann Marshak-Rothstein and Gregory A. Viglianti -- 23. Innate Immunity, Toll-Like Receptors, and Atherosclerosis: Mouse Models and Methods / Rosalinda Sorrentino and Moshe Arditi -- 24. Generation of Parasite Antigens for Use in Toll-Like Receptor Research / Philip Smith, Niamh B. Mangan and Padraic G. Fallon -- 25. Biomarkers Measuring the Activity of Toll-Like Receptor Ligands in Clinical Development Programs / Paul Sims, Robert L. Coffman and Edith M. Hessel.

ISBN

• 9781934115725 (hbk.)
• 193411572X (hbk.)

OCLC

• ocn261955183
• 261955183
• SCSB-5464272

Owning Institutions

Columbia University Libraries