Research Catalog

Title

Expressed sequence tags (ESTs) : generation and analysis / edited by John Parkinson.

Publication

Totowa, N.J. : Humana ; London : Springer [distributor], 2009.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Parkinson, John.

Description

x, 331 pages : illustrations; 27 cm.

Series Statement

• Methods in molecular biology, 1064-3745 ; 533
• Springer protocols

Uniform Title

• Methods in molecular biology.
• Springer protocols (Series)

Subjects

• Expressed Sequence Tags
• Nucleotide sequence > Methodology
• Base Sequence > methods

Bibliography (note)

• Includes bibliographical references and index.

Contents

1. Expressed Sequence Tags: An Overview / John Parkinson and Mark Blaxter -- 2. Strategies for Undertaking Expressed Sequence Tag (EST) Projects / Sandra W. Clifton and Makedonka Mitreva -- 3. Construction of cDNA Libraries: Focus on Protists and Fungi / Naiara Rodriguez-Ezpeleta, Shona Teijeiro, Lise Forget, Gertraud Burger and B. Franz Lang -- 4. Generation of Full-Length cDNA Libraries: Focus on Plants / Motoaki Seki, Asako Kamiya, Piero Carninci, Yoshihide Hayashizaki and Kazuo Shinozaki -- 5. Preparation of Full-Length cDNA Libraries: Focus on Metazoans / Masako Harada and Yoshihide Hayashizaki -- 6. Expressed Sequence Tags: Normalization and Subtraction of cDNA Libraries / Marcelo Bento Soares, Maria de Fatima Bonaldo, Jeremiah D. Hackett and Debashish Bhattacharya -- 7. Generating EST Libraries: Trans-Spliced cDNAs / Cecilia Fernandez and Rick M. Maizels -- 8. Large-Scale Sequencing and Analytical Processing of ESTs / Makedonka Mitreva and Elaine R. Mardis -- 9. EST Processing: From Trace to Sequence / Ralf Schmid and Mark Blaxter -- 10. Obtaining Accurate Translations from Expressed Sequence Tags / James Wasmuth and Mark Blaxter -- 11. EST Databases and Web Tools for EST Projects / Yao-Qing Shen, Emmet O'Brien, Liisa Koski, B. Franz Lang and Gertraud Burger -- 12. Phylogenomic Analysis of EST Datasets / Jose M. Peregrin-Alvarez and John Parkinson -- 13. Statistical Analysis of Expressed Sequence Tags / Edward Susko and Andrew J. Roger -- 14. Application of ESTs in Microarray Analysis / Weizhong Li, Lisa Olohan, Daryl Williams, Margaret Hughes, Andrew Gracey and Andrew Cossins -- 15. Exploiting ESTs in Human Health / Sandro Jose de Souza.

ISBN

• 9781588297594 (hbk.)
• 1588297594 (hbk.)

OCLC

• ocn276225718
• 276225718
• SCSB-5465349

Owning Institutions

Columbia University Libraries