Research Catalog

Title

Riboswitches : methods and protocols / edited by Alexander Serganov.

Publication

New York, NY : Humana, [2009], ©2009.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Serganov, Alexander.

Description

xi, 366 pages : illustrations; 27 cm.

Series Statement

• Methods in molecular biology, 1064-3745 ; v. 540
• Springer protocols

Uniform Title

• Methods in molecular biology (Clifton, N.J.) ; v. 540.
• Springer protocols (Series)

Subject

• RNA
• RNA-protein interactions
• Messenger RNA
• Ligands (Biochemistry)
• RNA > chemistry
• Gene Expression Regulation > physiology
• Clinical Laboratory Techniques

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

1. Predicting Riboswitch Regulation on a Genomic Scale / Jeffrey E. Barrick -- 2. Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemical Modifications / Renate Rieder, Claudia Hobartner and Ronald Micura -- 3. Application of Fluorescent Measurements for Characterization of Riboswitch-Ligand Interactions / Benoit Heppell, Jerome Mulhbacher, J. Carlos Penedo and Daniel A. Lafontaine -- 4. Transcriptional Approaches to Riboswitch Studies / Alexander Mironov, Vitaly Epshtein and Evgeny Nudler -- 5. Kinetics of Riboswitch Regulation Studied By In Vitro Transcription / J. Kenneth Wickiser -- 6. Molecular Basis of RNA-Mediated Gene Regulation on the Adenine Riboswitch by Single-Molecule Approaches / Jean-Francois Lemay, J. Carlos Penedo, Jerome Mulhbacher and Daniel A. Lafontaine -- 7. Methods for Analysis of Ligand-Induced RNA Conformational Changes / Chad A. Brautigam, Catherine A. Wakeman and Wade C. Winkler -- 8. Monitoring RNA-Ligand Interactions Using Isothermal Titration Calorimetry / Sunny D. Gilbert and Robert T. Batey -- 9. Preparation and Crystallization of Riboswitch-Ligand Complexes / Olga Pikovskaya, Artem A. Serganov, Ann Polonskaia, Alexander Serganov and Dinshaw J. Patel -- 10. Crystallization of the glmS Ribozyme-Riboswitch / Daniel J. Klein and Adrian R. Ferre-D'Amare -- 11. Riboswitch Conformations Revealed by Small-Angle X-Ray Scattering / Jan Lipfert, Daniel Herschlag and Sebastian Doniach -- 12. Time-Resolved NMR Spectroscopy: Ligand-Induced Refolding of Riboswitches / Janina Buck, Boris Furtig, Jonas Noeske, Jens Wohnert and Harald Schwalbe -- 13. Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2'-Hydroxyl Acylation and Primer Extension (SHAPE) / Catherine A. Wakeman and Wade C. Winkler -- 14. Identification of Metabolite-Riboswitch Interactions Using Nucleotide Analog Interference Mapping and Suppression / Juliane K. Soukup and Garrett A. Soukup -- 15. RNA-Dependent RNA Switches in Bacteria / Tina M. Henkin -- 16. Probing mRNA Structure and sRNA-mRNA Interactions in Bacteria Using Enzymes and Lead (II) / Clement Chevalier, Thomas Geissmann, Anne-Catherine Heifer and Pascale Romby -- 17. Structural Probing of RNA Thermosensors / Claude Chiaruttini, Frederic Allemand and Mathias Springer -- 18. Ribosomal Initiation Complexes Probed by Toeprinting and Effect of trans-Acting Translational Regulators in Bacteria / Pierre Fechter, Clement Chevalier, Gulnara Yusupova, Marat Yusupov, Pascale Romby and Stefano Marzi -- 19. Isolation and Characterization of the Heat Shock RNA 1 (HSR1) / Ilya Shamovsky and Fvgeny Nudler -- 20. Analysis of tRNA-Directed Transcription Antitermination in the T Box System in vivo / Tina M. Henkin -- 21. In vitro Selection of Conformational Probes for Riboswitches / Gunter Mayer and Michael Famulok -- 22. A Green Fluorescent Protein (GFP) Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo / Johannes H. Urban and Jorg Vogel -- 23. High-Throughput Screens to Discover Synthetic Riboswitches / Sean A. Lynch, Shana Topp and Justin P. Gallivan -- 24. A Mammalian Cell-Based Assay for Screening Inhibitors of RNA Cleavage / Laising Yen, Brent R. Stockwell and Richard C. Mulligan -- 25. In Vitro Selection of glmS Ribozymes / Kristian H. Link and Ronald R. Breaker.

ISBN

• 1934115886
• 9781934115886
• 9781597455589 (e-ISBN)
• 159745558X (e-ISBN)

LCCN

2008944038

OCLC

• ocn316002776
• 316002776
• SCSB-5470471

Owning Institutions

Columbia University Libraries