Research Catalog
Inositol phosphates and lipids : methods and protocols / edited by Christopher J. Barker.
- Title
- Inositol phosphates and lipids : methods and protocols / edited by Christopher J. Barker.
- Publication
- Totowa, N.J. : Humana ; London : Springer [distributor], 2010.
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| Status | Format | Access | Call Number | Item Location |
|---|---|---|---|---|
| Text | Request in advance | QH506 .M56 v.645 | Off-site |
Details
- Additional Authors
- Barker, Christopher J.
- Description
- x, 239 p. : ill.; 27 cm.
- Series Statement
- Methods in molecular biology ; 645
- Springer protocols
- Uniform Title
- Methods in molecular biology (Clifton, N.J.) ; v. 645.
- Springer protocols (Series)
- Subjects
- Genre/Form
- Laboratory Manual.
- Bibliography (note)
- Includes bibliographical references and index.
- Contents
- 1. The Role of Inositol and the Principles of Labelling, Extraction, and Analysis of Inositides in Mammalian Cells / Per-Olof Berggren -- 2. HPLC Separation of Inositol Polyphosphates / Per-Olof Berggren -- 3. Molecular Manipulation and Analysis of Inositol Phosphate and Pyrophosphate Levels in Mammalian Cells / John D. York -- 4. A Femtomole-Sensitivity Mass Assay for Inositol Hexakisphosphate / Robin F. Irvine -- 5. Synthesis of InsP, by the Inositol Hexakisphosphate Kinase I (IP6KI) / Adolfo Saiardi -- 6. Protein Pyrophosphorylation by Diphosphoinositol Pentakisphosphate (InsP7) / Rashna Bhandari -- 7. Synthesis and Nonradioactive Micro-analysis of Diphosphoinositol Phosphates by HPLC with Postcolumn Complexometry / Georg W. Mayr -- 8. Diphosphosinositol Polyphosphates and Energy Metabolism: Assay for ATP/ADP Ratio / Christopher Illies -- 9. Isolation of Inositol 1, 4, 5-Trisphosphate Receptor-Associating Proteins and Selective Knockdown Using RNA Interference / Katsuhiko Mikoshiba -- 10. Phosphoinositide-Specific Phospholipase C β l Signal Transduction in the Nucleus / Lucio Cocco -- 11. Methods to Assess Changes in the Pattern of Nuclear Phosphoinositides / Nullin Divecha -- 12. Measurement of Phosphoinositide 3-Kinase Products in Cultured Mammalian Cells by HPLC / Frank T. Cooke -- 13. Phosphoinositide Analysis by Liquid Chromatography-Mass Spectrometry / Trevor R. Pettitt -- 14. Imaging Phosphoinositide Dynamics in Living Cells / Anders Tengholm.
- ISBN
- 9781603271745 (hbk.)
- 1603271740 (hbk.)
- Owning Institutions
- Columbia University Libraries