Research Catalog

Title

Transcription factors : methods and protocols / edited by Paul J. Higgins.

Publication

Totowa, N.J. : Humana ; London : Springer [distributor], 2010.

Items in the Library & Off-site

Details

Additional Authors

Higgins, Paul J.

Description

xiv, 412 p. : ill.; 27 cm.

Series Statement

• Methods in molecular biology, 1940-6029 ; 647
• Springer protocols

Uniform Title

• Springer protocols (Series)
• Methods in molecular biology (Clifton, N.J.) ; v. 647.

Subject

• Transcription factors > Research > Methodology
• Transcription factors > Laboratory manuals
• Transcription Factors > analysis
• Sequence Analysis, DNA > methods
• Transfection > methods

Bibliography (note)

• Includes bibliographical references and index.

Contents

PART I. TRANSCRIPTION FACTOR TRAFFICKING AND POST-TRANSLATIONAL MODIFICATIONS: OVERVIEWS OF MECHANISMS -- 1. A Review of Post-translational Modifications and Subcellular Localization of Ets Transcription Factors: Possible Connection with Cancer and Involvement in the Hypoxic Response / Bohdan Wasylyk -- 2. Regulation of Transcription Factor Function by Targeted Protein Degradation: An Overview Focusing on p53, c-Myc, and c-Jun / Jukka Westermarck -- 3. Review of Molecular Mcchanisms Involved in the Activation of the Nrf2-ARE Signaling Pathway by Chemopreventive Agents / Maria C. Turco -- PART II. CYTOPLASMIC-NUCLEAR TRAFFICKING OF TRANSCRIPTION FACTORS -- 4. Subnuclear Localization and Intranuclear Trafficking of Transcription Factors / Gary S. Stein -- 5. Analysis of Ligand-Dependent Nuclear Accumulation of Smads in TGF-β Signaling / Xuedong Liu -- 6. Raf/MEK/MAPK Signaling Stimulates the Nuclear Translocation and Transactivating Activity of FOXMI / Kwok-Ming Yao -- 7. Coupling of Dephosphorylation and Nuclear Export of Smads in TGF-β Signaling / Xin-Hua Feng -- 8. Assessing Sequence-Specific DNA Binding and Transcriptional Activity of STATI Transcription Factor / Uwe Vinkemeier -- 9. Analysis of Nuclear Export Using Photoactivatable GFP Fusion Proteins and Interspecies Heterokaryons / Lina Dagnino -- 10. Determination of Nuclear Localization Signal Sequences for Kruppel-Like Factor 8 / Jihe Zhao -- 11. Methods to Measure Nuclear Export of β-Catenin Using Fixed and Live Cell Assays / Beric R. Henderson -- 12. Imaging of Transcription Factor Trafficking in Living Cells: Lessons from Corticosteroid Receptor Dynamics / Mayumi Nishi -- PART III. POST-TRANSLATIONAL MODIFICATIONS AND IMPACT ON FUNCTION -- 13. Hypoxia-Inducible Factors: Post-translational Crosstalk of Signaling Pathways / Thomas Kietzmann -- 14. The Basic Helix-Loop-Helix-Leucine Zipper Gene Mitf: Analysis of Alternative Promoter Choice and Splicing / Heinz Arnheiter -- 15. Phosphorylation Control of Nuclear Receptors / Cecile Rochette-Egly -- 16. Regulation of Krupple-Like Factor 5 by Targeted Protein Degradation / Ceshi Chen -- 17. Post-translational Control of ETS Transcription Factors: Detection of Modified Factors at Target Gene Promoters / Peter E. Shaw -- 18. Integration of Protein Kinases into Transcription Complexes: Identifying Components of Immobilised in Vitro Pre-initiation Complexes / Peter E. Shaw -- 19. Post-translational Modification of p53 by Ubiquitin / Chunhong Yan -- 20. Phosphorylation-Dependent Regulation of SATBI, the Higher-Order Chromatin Organizer and Global Gene Regulator / Sanjeev Galande -- PART IV. PROTOCOLS FOR OPTIMIZATION OF FUNCTIONAL ASSAYS -- 21. In Vivo and in Vitro Tools to Identify and Study Transcriptional Regulation of USF-1 Target Genes / Sebastien Corre -- 22. Measuring the Absolute Abundance of the Smad Transcription Factors Using Quantitative Immunoblotting / Xuedong Liu -- 23. Flow Cytometry Analysis of Transcription Factors in T Lymphocytes / Dorina Avram -- 24. Identification of Specific Protein/E-Box-Containing DNA Complexes: Lessons from the Ubiquitously Expressed USF Transcription Factors of the b-HLH-LZ Super Family / Yorann Baron.

ISBN

• 9781607617372 (hbk.)
• 1607617374 (hbk.)

OCLC

• ocn567148497
• 567148497
• SCSB-8855433

Owning Institutions

Columbia University Libraries