Research Catalog

Immunoelectron microscopy : methods and protocols / edited by Steven L. Schwartzbach, Testuaki Osafune.

Title
Immunoelectron microscopy : methods and protocols / edited by Steven L. Schwartzbach, Testuaki Osafune.

Items in the Library & Off-site

Filter by

1 Item

StatusFormatAccessCall NumberItem Location
Request for On-site UseRequest Scan
How do I pick up this item and when will it be ready?
TextRequest in advance QH506 .M56 v.657Off-site

Details

Additional Authors
  • Osafune, Tetsuaki.
  • Schwartzbach, Steven D.
Description
xi, 352 p. : ill.
Summary
"Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls" "Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships"--BOOK JACKET.
Series Statement
Methods in molecular biology 657
Subject
  • Microscopy, Electron
  • Microscopy, Immunoelectron
ISBN
  • 160761782X
  • 1607617838 (e-book)
  • 9781607617822
  • 9781607617839 (e-book)
Owning Institutions
Columbia University Libraries