Research Catalog

Title

Protein chromatography : methods and protocols / edited by Dermot Walls and Sinéad T. Loughran.

Publication

New York, N.Y. : Humana Press, c2011.

Items in the Library & Off-site

Details

Additional Authors

• Walls, Dermot
• Loughran, Sinéad T.

Description

x, 527 p. : ill. (some col.); 27 cm.

Series Statement

• Springer protocols
• Methods in molecular biology, 1940-6029 ; v. 681

Uniform Title

• Springer protocols (Series)
• Methods in molecular biology (Clifton, N.J.) ; v. 681.

Subject

• Proteomics
• Proteins > Analysis
• Chromatographic analysis
• Proteins > analysis
• Chromatography > methods

Bibliography (note)

• Includes bibliographical references and index.

Contents

A digest of protein purification -- Gel-filtration chromatography -- Immunoaffinity chromatography -- Avoiding proteolysis during protein chromatography -- Scale-up of protein purification : Downstream processing issues -- Phage display : A powerful technology for the generation of high specificity affinity reagents from alternative immune sources -- Engineering protein stability -- Microfluidics in protein chromatography -- Tagging recombinant proteins to enhance solubility and aid purification -- Storage and lyophilisation of pure proteins -- Differential precipitation and solubilization of proteins -- Ion-exchange chromatography : Basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase -- Protein quantitation and analysis of purity -- Purification of proteins fused to glutathione S-transferase -- Purification of proteins fused to maltose-binding protein -- Purification of proteins from baculovirus-infected insect cells -- Purification of poly-histidine-tagged proteins -- Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by cid/etd tandem mass spectrometry -- Tag removal by site-specific cleavage of recombinant fusion proteins -- Purification of antibodies using affinity chromatography -- Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens -- Measuring protein-protein interactions using biacore -- Ultra-performance liquid chromatography-mass spectrometry of proteins -- Hydrophobic interaction chromatography -- Fast protein liquid chromatography -- Shotgun proteomics : A qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS -- Shotgun proteomics : A relative quantitative approach using off-gel electrophoresis and LC-MS/MS -- Clinical proteomics : Liquid chromatography-mass spectrometry purification systems -- Strategies for the purification of membrane proteins -- A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases.

OCLC

• ocn680057439
• 680057439
• SCSB-8855495

Owning Institutions

Columbia University Libraries