Research Catalog

Protein chromatography : methods and protocols

Title
Protein chromatography : methods and protocols / edited by Dermot Walls and Sinéad T. Loughran.
Publication
New York, N.Y. : Humana Press, c2011.

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StatusFormatAccessCall NumberItem Location
Book/TextRequest in advance QH506 .M56 v.681Off-site

Details

Additional Authors
  • Walls, Dermot
  • Loughran, Sinéad T.
Description
x, 527 p. : ill. (some col.); 27 cm.
Series Statement
  • Springer protocols
  • Methods in molecular biology, 1940-6029 ; v. 681
Uniform Title
  • Springer protocols (Series)
  • Methods in molecular biology (Clifton, N.J.) ; v. 681.
Subject
  • Proteomics
  • Proteins > Analysis
  • Chromatographic analysis
  • Proteins > analysis
  • Chromatography > methods
Bibliography (note)
  • Includes bibliographical references and index.
Contents
A digest of protein purification -- Gel-filtration chromatography -- Immunoaffinity chromatography -- Avoiding proteolysis during protein chromatography -- Scale-up of protein purification : Downstream processing issues -- Phage display : A powerful technology for the generation of high specificity affinity reagents from alternative immune sources -- Engineering protein stability -- Microfluidics in protein chromatography -- Tagging recombinant proteins to enhance solubility and aid purification -- Storage and lyophilisation of pure proteins -- Differential precipitation and solubilization of proteins -- Ion-exchange chromatography : Basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase -- Protein quantitation and analysis of purity -- Purification of proteins fused to glutathione S-transferase -- Purification of proteins fused to maltose-binding protein -- Purification of proteins from baculovirus-infected insect cells -- Purification of poly-histidine-tagged proteins -- Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by cid/etd tandem mass spectrometry -- Tag removal by site-specific cleavage of recombinant fusion proteins -- Purification of antibodies using affinity chromatography -- Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens -- Measuring protein-protein interactions using biacore -- Ultra-performance liquid chromatography-mass spectrometry of proteins -- Hydrophobic interaction chromatography -- Fast protein liquid chromatography -- Shotgun proteomics : A qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS -- Shotgun proteomics : A relative quantitative approach using off-gel electrophoresis and LC-MS/MS -- Clinical proteomics : Liquid chromatography-mass spectrometry purification systems -- Strategies for the purification of membrane proteins -- A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases.
OCLC
  • ocn680057439
  • 680057439
  • SCSB-8855495
Owning Institutions
Columbia University Libraries