Research Catalog

Title

RNA : methods and protocols / edited by Henrik Nielsen.

Publication

New York, N.Y. : Humana Press, c2011.

Items in the Library & Off-site

Details

Additional Authors

Nielsen, Henrik.

Description

xv, 329 p. : ill.; 27 cm.

Series Statement

• Methods in molecular biology, 1940-6029 ; 703
• Springer Protocols.

Uniform Title

• Methods in molecular biology (Clifton, N.J.) ; v. 703.
• Springer protocols (Series)

Subject

• Messenger RNA
• Non-coding RNA
• RNA, Messenger
• RNA, Untranslated

Genre/Form

Laboratory Manual.

Bibliography (note)

• Includes bibliographical references and index.

Contents

The transcriptional landscape -- Working with RNA -- Synthesis of RNA by in vitro transcription -- Efficient poly(A)+ RNA selection using LNA oligo(T) capture -- Genome browsers -- Web-based tools for studying RNA structure and function -- Northern blotting analysis -- Rapid amplification of cDNA ends (RACE) -- Fractionation of mRNA based on the length of the poly(A) tail -- Analysis of mutations that influence pre-mRNA splicing -- S1 nuclease analysis of alternatively spliced mRNA -- Promises and challenges in developing RNAi as a research tool and therapy -- Inhibition of gene function in mammalian cells using short-hairpin RNA (shRNA) -- Validation of RNAi by real time PCR -- Profiling RNA polymerase II using the fast chromatin immunoprecipitation method -- The post-transcriptional operon -- RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray (chip) profiling -- Isolation of RNP granules -- Electrophoretic mobility shift assay for characterizing RNA-protein interaction -- Polysome analysis and RNA purification from sucrose gradients -- Prediction of targets for microRNAs -- Outsourcing of experimental work.

Owning Institutions

Columbia University Libraries