Research Catalog

Title

Genetic engineering / J. Williams, A. Ceccarelli and A. Wallace.

Author

Williams, J. G. (Jeff G.)

Publication

Oxford, UK : BIOS ; New York, NY : Springer, c2001.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Ceccarelli, A.
• Wallace, A. (Andrew), 1963-

Description

ix, 149 p. : ill. (some col.); 24 cm.

Series Statement

Medical perspectives series

Uniform Title

Medical perspectives series.

Subject

• Cloning, Organism > methods
• Escherichia coli > genetics
• Gene Expression > physiology
• Genetic Engineering > methods
• Genetic engineering

Bibliography (note)

• Includes bibliographical references and index.

Processing Action (note)

• committed to retain

Contents

1. Gene organization and expression -- General principles of gene organization and replication -- Common features of prokaryotic and eukaryotic gene expression -- Characteristic features of prokaryotic gene organization and expression -- Eukaryotic mRNA structure and post-transcriptional regulation -- Eukaryotic gene transcription and its regulation -- Transcriptional termination and 3' processing -- Splicing -- Chromosome structure -- 2. Gene analysis techniques I: low-resolution mapping of genomic DNA -- Nucleic acid hybridization -- Gel electrophoresis and detection of nucleic acids by Southern and Northern blotting -- Mapping genes to chromosomes -- Restriction enzyme mapping and its applications -- 3. Gene analysis techniques II: determining the structure of genes -- DNA sequence analysis -- Transcript mapping techniques -- Searching for genes using computers -- The polymerase chain reaction -- 4. Application of PCR technology to human diseases -- Genotyping using PCR technology -- Molecular analysis of clinical samples -- Diagnosing infectious diseases by PCR -- 5. Cloning in E. coli I: vectors and genomic libraries -- Cloning and cloning vectors -- Plasmid vectors and their use in manipulating DNA -- Phage vectors -- Construction and screening of genomic libraries -- 6. Cloning in E. coli II: isolating genes -- Construction of cDNA libraries -- Screening cDNA libraries -- Identifying genes by positional cloning: cystic fibrosis -- 7. Cloning in higher organisms -- Cloning in yeast -- Transformation of mammalian cells in tissue culture -- The creation and use of transgenic mice -- The impact of whole genome DNA sequence determination: proteomics and micro-arrays.

ISBN

0387915753

Owning Institutions

Harvard Library