Research Catalog

Title

Practical streptomyces genetics / Tobias Kieser ... [et al.].

Publication

Norwich : John Innes Foundation, 2000.

Items in the Library & Off-site

Holdings

Details

Additional Authors

• Kieser, Tobias
• Bibb, Mervyn J.
• Buttner, Mark J.
• Chater, K. F.
• Hopwood, D. A.
• John Innes Foundation issuing body.

Description

613 p. : ill.; 28 cm.

Summary

This entirely new practical guide and laboratory manual contains detailed protocols and hints for all aspects of streptomyces genetics, including over 100 pages of maps and detailed information on vectors and marker genes. It also extends into physiology, biochemistry and cytology, and is a rich source of background information about the biology of the of this group of mycelial, antibiotic-producing bacteria.

Subject

• Streptomyces > Laboratory manuals
• Bacterial genetics > Laboratory manuals

Genre/Form

Handbooks and manuals

Bibliography (note)

• Includes bibliographical references and index.

Processing Action (note)

• committed to retain

Contents

General introduction to actinomycete bioloyg -- Growth and preservation of streptomyces -- Microscopical methods -- Specialised biochemical techniques -- Mutagenesis of streptomyces by irradiation or chemicals -- Transportation mutagenesis in streptomyces -- In vivo genetic analysis by conjugation and protoplast fusion -- Preparation and analysis of genomic and plasmid DNA -- General considerations about gene cloning in streptomyces -- Introduction of DNA into streptomyces -- Plasmid and their use for gene cloning -- Streptomyces phages -- Cloning with phage vectors -- Gene disruption and gene replacement -- Reporter systems -- RNA methods -- Production and secretion of proteins by streptomyces -- Analysing streptomyces DNA -- Media, buffer and suppliers -- Genome maps and genetically marked strains -- Maps of DNA fragments -- Maps of plasmids, transposons and phage genomes.

ISBN

0708406238

OCLC

• 45236127
• SCSB-10118023

Owning Institutions

Harvard Library