Research Catalog
Practical streptomyces genetics / Tobias Kieser ... [et al.].
- Title
- Practical streptomyces genetics / Tobias Kieser ... [et al.].
- Publication
- Norwich : John Innes Foundation, 2000.
Items in the Library & Off-site
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Status | Vol/Date | Format | Access | Call Number | Item Location |
---|---|---|---|---|---|
2000 | Book/Text | Request in advance | QR82.S8 P72 2000 2000 | Off-site |
Holdings
Details
- Additional Authors
- Description
- 613 p. : ill.; 28 cm.
- Summary
- This entirely new practical guide and laboratory manual contains detailed protocols and hints for all aspects of streptomyces genetics, including over 100 pages of maps and detailed information on vectors and marker genes. It also extends into physiology, biochemistry and cytology, and is a rich source of background information about the biology of the of this group of mycelial, antibiotic-producing bacteria.
- Subject
- Genre/Form
- Handbooks and manuals
- Bibliography (note)
- Includes bibliographical references and index.
- Processing Action (note)
- committed to retain
- Contents
- General introduction to actinomycete bioloyg -- Growth and preservation of streptomyces -- Microscopical methods -- Specialised biochemical techniques -- Mutagenesis of streptomyces by irradiation or chemicals -- Transportation mutagenesis in streptomyces -- In vivo genetic analysis by conjugation and protoplast fusion -- Preparation and analysis of genomic and plasmid DNA -- General considerations about gene cloning in streptomyces -- Introduction of DNA into streptomyces -- Plasmid and their use for gene cloning -- Streptomyces phages -- Cloning with phage vectors -- Gene disruption and gene replacement -- Reporter systems -- RNA methods -- Production and secretion of proteins by streptomyces -- Analysing streptomyces DNA -- Media, buffer and suppliers -- Genome maps and genetically marked strains -- Maps of DNA fragments -- Maps of plasmids, transposons and phage genomes.
- ISBN
- 0708406238
- OCLC
- 45236127
- SCSB-10118023
- Owning Institutions
- Harvard Library