Research Catalog

Title

A manual for primary human cell culture / Jan-Thorsen Schantz, Kee Woel Ng.

Author

Schantz, Jan-Thorsten.

Publication

Singapore : World Scientific, c2010.

Items in the Library & Off-site

Details

Additional Authors

Ng, Kee-Woei.

Description

xxiii, 188 p. : ill. (some col.); 22 cm.

Summary

This manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its compact physical format that makes it portable and flexible for usage in a laboratory setting, the manual will be a useful guide for all beginners in primary human cell culture work.--Back cover.

Series Statement

Manuals in biomedical research ; vol. 6

Uniform Title

Manuals in biomedical research ; vol. 6.

Subject

• Cell Culture Techniques
• Human cell culture > Laboratory manuals

Genre/Form

• Laboratory Manual
• Laboratory manuals

Bibliography (note)

• Includes bibliographical references (p. [179]-180) and index.

Processing Action (note)

• committed to retain

Contents

A. Principles of biosafety -- A.1. Categories of biosafety -- A.2. Biohazard materials -- A.3. Recommended work practices -- A.4. General hygiene -- A.5. Handling biological specimens -- B. Introduction to the cell culture laboratory -- B.1. The laminar flow workbench -- biosafety cabinet -- B.2. The centrifuge -- B.3. The waterbath -- B.4. The sink/wet space -- B.5. The autoclave -- B.6. The refrigerator/freezer -- B.7. The incubator -- B.8. The microscope -- C. Protocols for human cell culture -- C.1. Cell isolation -- C.2. Mesenchymal cells -- C.3. Epithelial cells -- C.4. Bone marrow derived cells -- D. Reagents and techniques for cell culture -- D.1. Selection of cell culture media -- D.2. Cell culture reagents and media supplements -- D.3. Cell dissociation products -- E. Techniques for manipulating cell cultures and studying cellular behaviour -- E.1. Cryopreservation and thawing of cells -- E.2. Transfection -- E.3. Quantitative cell proliferation assays -- E.4. Assessment of gene expression -- F. Troubleshooting.

ISBN

• 9789812834775
• 981283477X

OCLC

• 687282347
• SCSB-10202729

Owning Institutions

Harvard Library