Research Catalog

Title

Signal transduction immunohistochemistry : methods and protocols / edited by Alexander E. Kalyuzhny.

Publication

• New York, NY : Humana Press, [2017]
• ©2017

Items in the Library & Off-site

Details

Additional Authors

Kalyuzhny, Alexander E.,

Description

x, 290 pages : illustrations; 26 cm.

Summary

This second edition volume provides detailed protocols that address the challenges of signal-transduction IHC. This book delves into chapters that discuss the nature of signal transduction phenomena and approaches to making phosphor-specific antibodies, as well as numerous bona fide methods on digital imaging techniques, preservation of tissue targets, multicolor detection, flow cytometry, lipophagy analysis, apoptosis, and the combination of IHC with in situ hybridization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and comprehensive, Signal Transduction Immunohistochemistry: Methods and Protocols, Second Edition is a valuable resource to both novices and experts in other fields of biomedical research who need advice on IHC protocols to study signal transduction. This book will also be useful for researchers in academia, government labs, and the biotech industry.

Series Statement

• Methods in molecular biology, 1064-3745 ; 1554
• Springer protocols

Uniform Title

• Methods in molecular biology (Clifton, N.J.) ; v. 1554,
• Springer protocols (Series)

Subject

• Cellular signal transduction
• Immunohistochemistry
• Immunology
• Medicine
• Signal Transduction > physiology

Genre/Form

Laboratory Manuals.

Bibliography (note)

• Includes bibliographical references and index.

Additional Formats (note)

• Also available in an electronic version.

Contents

Phosphosite-specific antibodies : a brief update on generation and applications / Kathy Brumbaugh [and four others] -- Antibodies that work again and again and again / Kameron Simpson and Michael Browning -- Validating antibody specificities for immunohistochemistry by protein blotting methods / Biji T. Kurien and R. Hal Scofield -- Acquisition and post-processing of immunohistochemical images / Jerry Sedgewick -- Using digital quantification of stained tissue microarrays as a medium-throughput, quantitative method for measuring the kinetics of signal transduction / Jessica Kalra and Jennifer BAker -- EicosaCel l: an imaging-based assay to identify spatiotemporal eicosanoid synthesis / Christianne Bandeira-Melo [and four others] -- Preservation of specific protein signaling states using heat based stabilizor system / Mats Borén -- Hapten-anti-hapten technique for two-color IHC detection of phosphorylated EGFR and H2AX using primary antibodies raised in the same host species / Jodi Hagen, David Schwartz, and Alexander E. Kalyuzhny -- Culture, transfection and immunocytochemical analysis of primary macrophages / Charles A. Warwick and Yuriy M. Usachev -- Phenotyping CD4+ hTh2 cells by flow cytometry : simultaneous detection of transcription factors, secreted cytokines, and surface markers / Chris Goetz [and three others] -- A coupled approach utilizing immunohistochemistry and immunocytochemistry to visualize cellular lipophagy / Aishwarya Sathyanarayan -- Apoptotic bodies : selective detection in extracellular vesicles / Paul Hauser, Sha Wang, and Vladimir V. Didenko -- Activation and differentiation of mesenchymal stem cells / Pravin J. Mishra and Debabrata Banjerjee -- High-sensitivity IHC detection of phosphorylated p27/Kip1 in human tissues using secondary antibody conjugated to polymer-HRP / Michsel Grahek, Ana Ptak, and Alexander E. Kalyuzhny -- Using phospho-peptides immobilized on magnetic beads for absorption control in immunohistochemistry / David Schwartz [and five others] -- Dual detection of nucleolytic and proteolytic markers of lysosomal cell death : DNase II-type breaks and Cathepsin D / Candace L. Minchew and Vladimir V. Didenko -- Multiplex immunohistochemistry for mapping the tumor microenvironment / Jessica Kalra and Jennifer Baker -- Validation of anti-mouse PDL-1 goat polyclonal antibody staining with mouse PDL-1 in situ hybridization on adjacent sections of cell pellets and mouse tumors / Jacob Ricca [and nine others] -- Automated double in situ detection of mouse Lgr5 mRNA and lysozyme protein in examining the neighboring cell types of the mouse intestinal crypt / Mesruh Turkekul [and six others] -- Automated high-content screening of [gamma]-H2AX expression in HeLa cells / Nate Hopp [and three others].

ISBN

• 1493967576
• 9781493967599 (eBook) (canceled/invalid)

LCCN

• 9781493967575
• 2016962312

Owning Institutions

Princeton University Library