Research Catalog

Title

Argonaute proteins : methods and protocols / edited by Katsutomo Okamura, Kotaro Nakanishi.

Publication

New York : Humana Press, [2018]

Items in the Library & Off-site

Details

Additional Authors

• Nakanishi, Kotaro,
• Okamura, Katsutomo,

Description

xiii, 260 pages : illustrations (some color); 26 cm.

Series Statement

• Methods in molecular biology ; 1680
• Springer Protocols

Uniform Title

• Methods in molecular biology (Clifton, N.J.) ; 1680.
• Springer protocols (Series)

Subjects

• RNA-protein interactions > Laboratory manuals
• RNA interference > Laboratory manuals

Bibliography (note)

• Includes bibliographical references and index.

Additional Formats (note)

• Also available in an electronic version.

Contents

Cloning and identification of recombinant argonaute-bound small RNAs using next-generation sequencing / Pooja Gangras, Daniel M. Dayeh, Justin W. Mabin, Kotaro Nakanishi, and Guramrit Singh -- Quantification of miRNAs co-immunoprecipitated with argonaute proteins using SYBR green-based qRT-PCR / Hong-Duc Phan, Junan Li, Ming Poi, and Kotaro Nakanishi -- Gateway to understanding argonaute loading of single-stranded RNAs : preparation of deep sequencing libraries with in vitro loading samples / Eling Goh and Katsutomo Okamura -- Dumbbell-PCR for discriminative quantification of a small RNA variant / Megumi Shigematsu, Shozo Honda, and Yohei Kirino -- MicroRNA detection by whole-mount in situ hybridization in C. elegans / Yoshiki Andachi and Yuji Kohara -- cCLIP-seq : retrieval of chimeric reads from HITS-CLIP (CLIP-Seq) libraries / Panagiotis Alexiou, Manolis Maragkakis, Zissimos Mourelatos, and Anastassios Vourekas -- Kinetic analysis of small silencing RNA production by human and drosophila dicer enzymes in vitro / Susan E. Liao and Ryuya Fukunaga -- Nucleic acid-binding assay of argonaute protein using fluorescence polarization / Tomohiro Miyoshi -- Reconstitution of RNA interference machinery / Shintaro Iwasaki and Yukihide Tomari -- Single-molecule analysis for RISC assembly and target cleavage / Hiroshi M. Sasaki, Hisashi Tadakuma, and Yukihide Tomari -- Profiling open chromatin structure in the ovarian somatic cells using ATAC-seq / Kensaku Murano, Yuka W. Iwasaki, and Haruhiko Siomi -- Assessing miR-451 activity and its role in erythropoiesis / Dmitry A. Kretov, Andrew M. Shafik, and Daniel Cifuentes -- Functional analysis of microRNAs in neurogenesis during mouse cortical development / Wei Zhang, Xiaoxia Zeng, and Li Zeng -- Cellular approaches in investigating argonaute2-dependent RNA silencing / Cai Zhang, Joonbae Seo, and Takahisa Nakamura -- Genomic tagging of AGO1 using CRISPR/Cas9-mediated homologous recombination / Sanjay Ghosh and Ji-Long Liu -- Accurate profiling and quantification of tRNA fragments from RNA-Seq data : a Vade Mecum for MINTmap / Phillipe Loher, Aristeidis G. Telonis, and Isidore Rigoutsos.

ISBN

• 9781493973385
• 149397338X

Owning Institutions

Princeton University Library