Research Catalog

Title

Duchenne muscular dystrophy : methods and protocols / edited by Camilla Bernardini.

Publication

• New York : Humana Press, [2018]
• ©2018

Items in the Library & Off-site

Details

Additional Authors

Bernardini, Camilla,

Description

xii, 287 pages : illustrations (some color); 27 cm.

Summary

"This volume explores experimental approaches used to study Duchenne muscular dystrophy (DMD), an X-linked degenerative skeletal muscle disease caused by mutations in the dystrophin gene. Including the latest progress and scientific achievements, the book covers recent discoveries achieved through in vivo gene editing which have proven to be promising in restoring dystrophin expression, at least in ameliorating skeletal muscle symptoms, and the contents focus on "Omics" techniques in gene expression, protein expression, miRNAs, and long non-coding RNA analysis, as well as experimental studies of the structural/functional changes affecting the skeletal and cardiac muscles and ongoing preclinical studies and clinical trials. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Duchenne Muscular Dystrophy: Methods and Protocols serves as a guide for researchers exploring the complicated nature of dystrophin in the hope of helping the victims of this disorder"--Publisher's description.

Series Statement

• Methods in molecular biology, 1064-3745 ; v. 1687
• Springer protocols

Uniform Title

• Methods in molecular biology (Clifton, N.J.) ; v. 1687,
• Springer protocols (Series)

Subject

• Duchenne muscular dystrophy > Laboratory manuals
• Duchenne muscular dystrophy
• Dystophin > genetics
• Muscular Dystrophy, Duchenne

Genre/Form

• Laboratory Manuals.
• Laboratory manuals.

Bibliography (note)

• Includes bibliographical references and index.

Contents

Overview of recent therapeutics advances for duchenne muscular dystrophy / Jean K. Mah -- Clinical manifestations and overall management strategies for duchenne muscular dystrophy / Takeshi Tsuda -- Cardiac involvement in duchenne muscular dystrophy and related dystrophinopathies / Sophie I. Mavrogeni, George Markousis-Mavrogenis, Antigoni Papavasiliou, George Papadopoulos, and Genovefa Kolovou -- Characterization of the inflammatory response in dystrophic muscle using flow cytometry / Jenna M. Kastenschmidt, Ileen Avetyan, and S. Armando Villalta -- Imaging analysis of the neuromuscular junction in dystrophic muscle / Stephen J.P. Pratt, Shama R. Iyer, Sameer B. Shah, and Richard M. Lovering -- System biology approach : gene network analysis for muscular dystrophy / Federica Censi, Giovanni Calcagnini, Eugenio Mattei, and Alessandro Giuliani -- Proteomic profiling of the dystrophin-deficient brain / Sandra Murphy and Kay Ohlendieck -- Probing the pathogenesis of duchenne muscular dystrophy using mouse models / Alexander Morrison-Nozik and Saptarsi M. Haldar -- Exon skipping therapy using phosphorodiamidate morpholino oligomers in the mdx52 mouse model of duchenne muscular dystrophy / Shouta Miyatake, Yoshitaka Mizobe, Hotake Takizawa, Yuko Hara, Toshifumi Yokota, Shinichi Takeda, and Yoshitsugu Aoki -- Designing effective antisense oligonucleotides for exon skipping / Takenori Shimo, Rika Maruyama, and Toshifumi Yokota -- Identification of splicing factors involved in DMD exon skipping events using an in vitro RNA binding assay / Julie Miro, Cyril F. Bourgeois, Mireille Claustres, Michel Koenig, and Sylvie Tuffery-Giraud -- Use of antisense oligonucleotides for the treatment of duchenne muscular dystrophy / Karima Relizani and Aurelie Goyenvalle -- PMO delivery system using bubble liposomes and ultrasound exposure for duchenne muscular dystrophy treatment / Yoichi Negishi, Yuko Ishii, Kei Nirasawa, Eri Sasaki, Yoko Endo-Takahashi, Ryo Suzuki, and Kazuo Maruyama -- Proton nuclear magnetic resonance (1H NMR) spectroscopy-based analysis of lipid components in serum/plasma of patients with duchenne muscular dystrophy (DMD) / Niraj Kumar Srivastava -- Test of antifibrotic drugs in a cellular model of fibrosis based on muscle-derived fibroblasts from duchenne muscular dystrophy patients / Simona Zanotti and Marina Mora -- Flow cytometry-defined CD49d expression in circulating T-lymphocytes is a biomarker for disease progression in duchenne muscular dystrophy / Wilson Savino, Fernanda Pinto-Mariz, and Vincent Mouly -- Advanced methods to study the cross talk between fibro-adipogenic progenitors and muscle stem cells / Luca Tucciarone, Usue Etxaniz, Martina Sandona , Silvia Consalvi, Pier Lorenzo Puri, and Valentina Saccone -- AAV6 vector production and purification for muscle gene therapy / Christine L. Halbert, James M. Allen, and Jeffrey S. Chamberlain -- From gRNA identification to the restoration of dystrophin expression : a dystrophin gene correction strategy for duchenne muscular dystrophy mutations using the CRISPR-induced deletion method / Benjamin Duchene, Jean-Paul Iyombe-Engembe, Joel Rousseau, Jacques P. Tremblay, and Dominique L. Ouellet.

ISBN

• 1493973738
• 9781493973743 (canceled/invalid)
• 1493973746 (canceled/invalid)

Owning Institutions

Princeton University Library