Research Catalog
RNA interference (RNAi) : nuts & bolts of RNAi technology /
- Title
- RNA interference (RNAi) : nuts & bolts of RNAi technology / edited by David R. Engelke.
- Publication
- [Eagleville, PA] : DNA Press, c2003.
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| Status | Format | Access | Call Number | Item Location |
|---|---|---|---|---|
Not available - Please for assistance. | Text | Use in library | QH450 .R629 2003 | Off-site |
Details
- Additional Authors
- Engelke, David R. (David Ross)
- Description
- viii, 236 p. : ill.; 22 cm.
- Series Statement
- The nuts & bolts series
- Alternative Title
- Ribonucleic acid interference (RNAi)
- Subject
- Bibliography (note)
- Includes bibliographical references and index.
- Contents
- Section I. RNA interference as an experimental tool in invertebrates. -- Chapter 1. Targeted gene silencing in plants using RNA interference -- Chapter 2. RNAi in caenorhabditis elegans -- Chapter 3. Application and analysis of RNAi in drosophila systems -- Section II. Short interfering RNA as a tool in vertebrate systems: synthesizing the RNA -- Chapter 4. Gene silencing by synthetic siRNA duplexes in mammalian cell culture -- Chapter 5. Strategies for synthesizing small interfering RNA (siRNA) -- Chapter 6. Generating long dsRNA, individual siRNA and siRNA cocktails in vitro -- Section III. Short interfering RNA as a tool in vertebrate systems: applications -- Chapter 7. Optimizing high throughput RNAi-based assays using transient transfection of synthetic siRNAs in cultured mammalian cells -- Chapter 8. Subcellular delivery and detection of small interfering RNA -- Chapter 9. Downregulation of cellular genes by PCR products expressing siRNAs mammalian cells -- Chapter 10. Generating adenoviruses for shRNA or siRNA delivery -- Chapter 11. Achieving stable, heritable gene silencing in the mouse.
- ISBN
- 0966402782 (alk. paper)
- Owning Institutions
- Princeton University Library