Research Catalog

Title

Hepatitis B virus : methods and protocols / edited by Haitao Guo, Andrea Cuconati.

Publication

• New York, NY : Humana Press, [2017]
• ©2017

Items in the Library & Off-site

Details

Additional Authors

• Guo, Haitao (Associate professor)
• Cuconati, Andrea
• Springer Science+Business Media, copyright holder. cph

Description

xii, 297 pages : illustrations (some color), still photographic images; 26 cm.

Series Statement

• Methods in molecular biology, 1064-3745 ; 1540
• Springer protocols

Uniform Title

• Methods in molecular biology (Clifton, N.J.) ; v. 1540.
• Springer protocols (Series)

Subject

• Hepatitis B virus > Laboratory manuals
• Hepatitis B virus
• Hepatitis B virus
• Virus de l'hépatite B > Manuels de laboratoire
• Virus de l'hépatite B

Genre/Form

• Laboratory Manual
• Laboratory manuals.
• Manuels de laboratoire.

Bibliography (note)

• Includes bibliographical references andb index.

Contents

NTCP-Reconstituted In Vitro HBV Infection System -- Hepatitis B Virus Infection of HepaRG Cells, HepaRG-hNTCP Cells and Primary Human Hepatocytes -- Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells -- Intracytoplasmic Transport of Hepatitis B Virus Capsids -- A Homokaryon Assay for Nucleocytoplasmic Shuttling Activity of HBV Core Protein -- Analyses of HBV cccDNA Quantification and Modification -- Detection of HBV cccDNA Methylation from Clinical Samples by Bisulfite Sequencing and Methylation Specific PCR -- A T7 Endonuclease I Assay to Detect TALEN-Mediated Mutation of HBV cccDNA -- Detection of Hepatocyte Clones Containing Integrated Hepatitis B Virus DNA using Inverse Nested PCR -- Highly Sensitive Detection of HBV RNA in Liver Tissue by In Situ Hybridization -- Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice -- Measuring Changes in Cytosolic Calcium Levels in HBV- and HBx-Expressing Cultured Primary Hepatocytes -- In Vitro Assays for RNA Binding and Protein Priming of Hepatitis B Virus Polymerase -- In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV RNaseH Inhibitors -- Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay -- Microtiter-Format Assays for HBV Antigen Quantitation in Non-Clinical Applications -- Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform -- Generation of Replication Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization -- Hydrodynamic HBV Transfection Mouse Model -- An ELISPOT-Based Assay to Measure HBV-Specific CD8+ T Cell Responses in Immunocompetent Mice -- Advanced Method for Isolation of Mouse Hepatocytes, Liver Sinusoidal Endothelial Cells and Kupffer Cells -- Partial Hepatectomy and Castration of HBV Transgenic Mice -- Studying HBV Infection and Therapy in Immune Deficient NOD-Rag1-/-IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (Fah) Knockout Mice Transplanted with Human Hepatocytes -- Measurement of Antiviral Effect and Innate Immune Response during Treatment of Primary Woodchuck Hepatocytes.

ISBN

• 1493966987
• 9781493966981
• 1493967002 (canceled/invalid)
• 9781493967001 (canceled/invalid)

LCCN

2106961703

OCLC

• ocn957533331
• 957533331
• SCSB-1882194

Owning Institutions

Princeton University Library