Research Catalog

Title

mRNA processing : methods and protocols.

Publication

New York, NY : Humana Presss, 2017.

Items in the Library & Off-site

Holdings

Details

Additional Authors

Shi, Yongsheng edt

Description

xi, 258 pages: illustrations; 25 cm.

Series Statement

• Methods in molecular biology ; 1648
• Springer protocols

Uniform Title

• Methods in molecular biology (Clifton, N.J.) ; 1648.
• Springer protocols (Series)

Subject

Messenger RNA > Laboratory manuals

Additional Formats (note)

• Also available in an electronic version.

Contents

Detection and identification of uncapped RNA by ligation-mediated reverse transcription polymerase chain reaction / C. Kiong Ho -- Preparation of splicing competent nuclear extract from mammalian cells and in vitro pre-mRNA splicing assay / Maliheh Movassat, Hossein Shenasa, and Klemens J. Hertel -- Single-molecule analysis of pre-mRNA splicing with colocalization single-molecule spectroscopy (CoSMoS) / Joerg E. Braun and Victor Serebrov -- In vitro modulation of endogenous alternative splicing using splice-switching antisense oligonucleotides / Jeong Eun Park and Luca Cartegni -- Purification of mRNA processing complexes using an RNA affinity approach / Xiuye Wang and Yongsheng Shi -- Polyadenylation site-based analysis of transcript expression by 3'READS+ / Dinghai Zheng and Bin Tian -- Comprehensive identification of mRNA polyadenylation sites by PAPERCLIP / Hun-Way Hwang and Robert B. Darnell -- Microinjection and fluorescence in situ hybridization assay for studying mRNA export in mammalian cells / Ke Wang, Min Shi, and Hong Cheng -- Methods for the detection of adenosine-to-inosine editing events in cellular RNA / Eimile Oakes, Pranathi Vadlamani, and Heather A. Hundley -- Using RNA-Seq to discover genetic polymorphisms that produce hidden splice variants / Shayna Stein, Emad Bahrami-Samani, and Yi Xing -- Inducible expression of eukaryotic circular RNAs from plasmids / Deirdre C. Tatomer, Dongming Liang, and Jeremy E. Wilusz -- Methods for extraction of RNA, proteins, or protein complexes from subcellular compartments of eukaryotic cells / Kyu-Hyeon Yeom and Andrey Damianov -- Isolation of newly transcribed RNA using the metabolic label 4-thiouridine / Angela Garibaldi, Francisco Carranza, and Klemens J. Hertel -- Robust, cost-effective profiling of RNA binding protein targets with single-end enhanced crosslinking and immunoprecipitation (seCLIP) / Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, and Gene W. Yeo -- Purification of transcript-specific mRNP complexes formed in vivo from Saccharomyces cerevisiae / Jenna E. Smith and Kristian E. Baker -- Cell-based high-throughput method for identifying modulators of alternative splicing / Sika Zheng -- Genome-wide RNAi screens for RNA processing events in Drosophila melanogaster S2 cells / Natoya Peart and Eric J. Wagner -- Assaying RNA structure inside living cells with SHAPE / Chao Feng, Dalen Chan, and Robert C. Spitale.

ISBN

• 9781493972036
• 1493972030

OCLC

• ocn985081643
• SCSB-8846497

Owning Institutions

Princeton University Library