Research Catalog

Title

Sphingosine-1-phosphate : methods and protocols / edited by Alice Pébay, Kursad Turksen.

Publication

• New York, NY : Humana Press, [2018]
• ©2018

Items in the Library & Off-site

Details

Additional Authors

• Pébay, Alice
• Turksen, Kursad

Description

x, 182 pages : illustrations (some color); 26 cm.

Summary

This fully updated edition explores methods involving sphingosine-1-phosphate (S1P), a bioactive lysophospholipid which has become the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells. Here, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. As a volume in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols, Second Edition is another key resource for scientists working in this fast-moving and dynamic field.

Series Statement

• Methods in molecular biology, 1064-3745 ; 1697
• Springer protocols

Uniform Title

• Methods in molecular biology (Clifton, N.J.) ; v. 1697.
• Springer protocols (Series)

Subject

• Sphingosine > Laboratory manuals
• Sphingolipids
• Sphingosine
• Sphingolipids
• Sphingosine

Genre/Form

• Laboratory Manual
• Laboratory manuals
• Laboratory manuals.
• Manuels de laboratoire.

Bibliography (note)

• Includes bibliographical references and index.

Contents

Measuring Sphingosine-1-Phosphate/Protein Interactions with the Kinetic Exclusion Assay -- An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity -- Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells -- Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry -- Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines -- A Clean-Up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule -- A Rapid Fluorescence Assay for Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes -- Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-Parametric Flow Cytometry -- S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses -- In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate -- Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor -- Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors -- Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation -- 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering.

ISBN

• 9781493974122
• 1493974122
• 9781493974139 (eBook) (canceled/invalid)

LCCN

2017959561

OCLC

• ocn994729493
• 994729493
• SCSB-8956774

Owning Institutions

Princeton University Library