Research Catalog

Title

Tissue in situ hybridization : methods in animal development / Trevor Jowett.

Author

Jowett, Trevor.

Publication

New York : Wiley ; Heidelberg : Spektrum, ©1997.

Items in the Library & Off-site

Details

Description

viii, 128 pages, 8 pages of plates : illustrations (some color); 26 cm.

Summary

"The European Molecular Biology Organization (EMBO) course on tissue in situ hybridization in animal developmental biology has served as an important and highly respected forum for the latest advance in the methodology of this valuable research tool. Developed from EMBO course materials, Tissue In Situ Hybridization provides scientists and researchers worldwide with detailed coverage of new approaches, techniques, and protocols, along with up-to-date information on more established procedures."--BOOK JACKET. "Focusing particularly on the two-color in situ hybridization method to whole-mount embryos and tissue sections, this practical resource also compares different methods of producing differentially colored signals, and includes the result of protocol experiments with fluorescent and other alternative in situ hybridization techniques."--BOOK JACKET.

Series Statement

An EMBO practical course

Uniform Title

EMBO practical course

Subject

• Animal Bildband
• In situ hybridization
• Developmental cytology
• Developmental biology
• In Situ Hybridization
• Developmental Biology
• Developmental biology
• Developmental cytology
• In situ hybridization
• Gewebe
• Hybridisierung Biologie
• In-situ-Hybridisierung
• Hybridation in situ
• Cytologie du développement

Note

• "An EMBO practical course"--Cover.

Bibliography (note)

• Includes bibliographical references (p. [119]-121) and index.

Contents

Ch. 1. Principle of the Technique -- Ch. 2. Probe Synthesis -- Ch. 3. Fixation -- Ch. 4. Hybridization to Whole-Mount Embryos -- Ch. 5. Immunolocalization of Probes -- Ch. 6. Alternative Enzyme Substrates -- Ch. 7. Two-Color In situ Hybridizations -- Ch. 8. Two-Color In situ Hybridization with Fluorochromes -- Ch. 9. Embedding and Sectioning Whole-Mount Embryos -- Ch. 10. In situ Hybridization on Paraffin Wax Embedded Tissue Sections -- Ch. 11. In situ Hybridization on Cryostat Sections of Chick Embryos -- Ch. 12. Whole-Mount In situ Hybridization of Thick, Floating Tissue Sections -- Ch. 13. Simultaneous Localization of Transcription and Translation Gene Products -- Ch. 14. Special Considerations, Modifications, and Problem Troubleshooting -- Ch. 15. Photomicrography -- Ch. 16. Solutions, Reagents, Equipment, and Suppliers.

ISBN

• 0471164038
• 9780471164036

LCCN

96035310

OCLC

• ocm35567018
• 35567018
• SCSB-9624162

Owning Institutions

Princeton University Library