Research Catalog
Cell-free gene expression : methods and protocols
- Title
- Cell-free gene expression : methods and protocols / edited by Ashty S. Karim, Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, and Center for Synthetic Biology, Northwestern University, Evanston, IL, USA, Michael C. Jewett, Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, Center for Synthetic Biology, Robert H. Lurie Comprehensive Cancer Center, and Simpson Querry Institute, Northwestern University, Evanston, IL, USA.
- Publication
- New York, NY : Humana Press, [2022]
- ©2022
Items in the Library & Off-site
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1 Item
| Status | Format | Access | Call Number | Item Location |
|---|---|---|---|---|
| Text | Use in library | QH506 .M45 1984 vol.2433 | Off-site |
Details
- Additional Authors
- Description
- xv, 435 pages : illustrations (chiefly colour); 26 cm.
- Summary
- "This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell-Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications."--
- Series Statement
- Methods in molecular biology, 1064-3745 ; 2433
- Springer protocols
- Uniform Title
- Methods in molecular biology ; v. 2433.
- Springer protocols (Series)
- Subject
- Bibliography (note)
- Includes bibliographical references and index.
- Terms of Use (note)
- Current copyright fee: GBP19.00
- ISBN
- 9781071619971
- 1071619977
- 9781071619988 (canceled/invalid)
- 1071619985 (canceled/invalid)
- OCLC
- on1285694207
- SCSB-14108772
- Owning Institutions
- Princeton University Library