Research Catalog

Title

Lipid analysis; isolation, separation, identification, and structural analysis of lipids [by] William W. Christie.

Author

Christie, William W.

Publication

Oxford, New York, Pergamon Press [1973]

Items in the Library & Off-site

Details

Description

xiv, 338 pages illustrations; 22 cm

Subject

• Lipids > Analysis
• Lipids > analysis
• Analyse
• Lipide

Bibliography (note)

• Includes bibliographies.

Contents

The structure, chemistry and occurrence of lipids : The fatty acids : Saturated fatty acids ; Monoenoic fatty acids ; Non-conjugated polyunsaturated fatty acids ; Branched-chain and cyclopropane fatty acids ; Unusual fatty acids of plant origin. Simple lipids : Triglycerides and partial glycerides ; Alkyl diglycerides and neutral plasmalogens ; Cholesterol and cholesteryl ; Wax esters and other minor components. Complex lipids : Phosphoglycerides ; Glycosyl diglycerides and related compounds ; Sphingolipids. Structural features of lipids important in analyses -- The isolation of lipids from tissues : General principles of solvent extraction procedures : Storage of tissues ; The solubility of lipids in organic solvents ; Removal of non-lipid contaminants ; Artefacts of extraction procedures ; Some practical considerations. Recommended procedures : Extraction of large amounts of tissue ; Chloroform-methanol (2:1, v/v) extraction and “Folch” wash ; Sephadex G-25 columns for removing non-lipid contaminants -- Chromatographic and spectroscopic analysis of lipids, general principles : A statement of the problem ; Chromatographic procedures : Gas-liquid chromatography ; Adsorption chromatography ; Diethylaminoethyl (DEAE) cellulose chromatography ; Partition chromatography ; Chromatography on adsorbents containing complexing agents. Some practical considerations : Autoxidation of lipids ; Solvents ; Contaminants. Spectroscopy : Infrared absorption spectroscopy ; Ultraviolet absorption spectroscopy ; Nuclear magnetic resonance spectroscopy ; Mass spectroscopy ; Optical rotary dispersion. Equipping a laboratory for lipid analysis -- The preparation of volatile derivatives of lipids : Saponification of lipids ; The preparation of methyl esters of fatty acids : Acid-catalysed esterification and transesterification ; Base-catalysed transesterification ; Diazomethane ; Special cases. Derivatives of free hydroxyl groups : Acetylation ; Trifluoroacetates ; Trimethylsilyl ethers ; Isopropylidene compounds ; n-Butylboronate derivatives. Derivatives of fatty aldehydes : Hydrazone derivatives ; Acetals. Derivatives of double bonds : Mercuric acetate derivatives ; Hydroxylation ; Epoxidation ; Deuterohydrazine reduction -- The analysis of fatty acids : Analytical gas-liquid chromatography : Fatty acids of animal origin ; Unusual fatty acids ; Spurious peaks on recorder traces. Isolation of individual fatty acids for structural analysis : Adsorption chromatography ; Silver nitrate chromatography ; Preparative gas chromatography ; Liquid-liquid partition chromatography. Spectroscopy of fatty acids : Infrared absorption spectroscopy ; Ultraviolet spectroscopy ; Nuclear magnetic resonance spectroscopy ; Mass spectrometry. Hydrogenation ; Location of double bonds in fatty acid claims : Permanganate-periodate oxidation ; Ozonolysis and reductive cleavage ; Oxidation after partial reduction of polyunsaturated acids. Location of other functional groups in fatty acids ; Physical characterisation of fatty acids ; Preparation of large quantities of pure fatty acids : Saturated fatty acids ; Polyunsaturated fatty acids -- The analysis of simple lipid classes : Chromatographic separation of the common simple lipid classes : Thin-layer chromatography ; Column chromatography ; Gas-liquid chromatography ; Separation of isomeric mono- and diglycerides. Analysis of the hydrolysis products of simple lipids : Fatty acids ; Glycerol ; Cholesterol. Alkyl diglycerides and neutral plasmalogens : Separation and identification ; Isolation and identification of aldehydes from plasmalogens ; Isolation and identification of ɪ-alkyl glycerols. Wax esters and related compounds -- The analysis of complex lipids : Preliminary separation and preparation of lipid samples : Simple group separations ; Cations associated with complex lipids. Column chromatography : Silicic acid ; Florisil ; Alumina ; DEAE and TEAE cellulose chromatography. Thin-layer chromatography : Single-dimensional TLC systems ; Two-dimensional TLC systems ; Location and identification of complex lipids on TLC plates. Determination of complex lipids separated by chromatographic procedures ; Analysis of complex lipids as their partial hydrolysis products ; Sphingolipids : Cerebrosides and ceramide polyhexosides ; Gangliosides ; Long-chain bases. Alkyl- and alkenyl-ether derivatives of complex lipids ; Phosphonolipids ; Determination of water-soluble hydrolysis products of complex lipids : Phosphorus ; Glycerol ; Carbohydrates, N-acetylneuraminic acid and related compounds ; Nitrogenous bases of phospholipids -- The analysis of molecular species of lipids : General methods of analysis : Liquid-solid and liquid-liquid chromatography ; Gas-liquid chromatography of intact lipids. Molecular species of simple lipids : Cholesteryl esters ; Monoglycerides ; Diglycerides ; Triglycerides ; Wax esters. Molecular species of complex lipids : General approaches to the problem ; Phosphatidyl choline ; Phosphatidyl ethanolamine ; Other glycerophosphatides; Glycosyl diglycerides ; Sphingomyelin and glycosphingolipids -- Enzymatic hydrolysis of lipids : Positional distribution of fatty acids in triglycerides : Pancreatic lipase hydrolysis ; Stereospecific analysis of triglycerides. Enzymatic hydrolysis of complex lipids : Phospholipase A2 ; Pancreatic lipase and related enzymes ; Phospholipase C ; Phospholipase D. The use of enzymatic hydrolysis in the determination of molecular species of lipids -- The analysis and radioassay of isotopically-labelled lipids : Thin-layer chromatography ; Gas-liquid chromatography : Radioassay after preparative GLC ; Continuous monitoring of the column effluent. Location of ¹⁴C in aliphatic chains -- A summary : Extraction of lipids from tissues ; Fatty acid compositions ; The analysis of simple lipids ; The analysis of complex lipids ; Structural analysis of lipids.

ISBN

• 0080177530
• 9780080177533

LCCN

73008946

OCLC

• ocm00640951
• 640951
• SCSB-297846

Owning Institutions

Princeton University Library