Research Catalog
Ultrasensitive and rapid enzyme immunoassay
- Title
- Ultrasensitive and rapid enzyme immunoassay / Eiji Ishikawa.
- Author
- Ishikawa, Eiji, 1933-
- Publication
- Amsterdam ; New York : Elsevier, 1999.
Items in the Library & Off-site
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1 Item
| Status | Format | Access | Call Number | Item Location |
|---|---|---|---|---|
| Text | Use in library | 8617.985 vol.27 | Off-site |
Details
- Description
- xvi, 334 pages : illustrations; 20 cm
- Summary
- Immunoassay techniques have become essential in various fields of pure and applied research. This guide describes many of the techniques used in this field, how to avoid and overcome obstacles encountered, and some of the theory behind the methods.
- Series Statement
- Laboratory techniques in biochemistry and molecular biology ; v. 27
- Uniform Title
- Laboratory techniques in biochemistry and molecular biology ; v. 27.
- Subject
- Genre/Form
- Laboratory Manual
- Laboratory manuals
- Laboratory manuals.
- Manuels de laboratoire.
- Note
- Series title appears on spine.
- Bibliography (note)
- Includes bibliographical references (p. 311-325) and index.
- Contents
- 1. Introduction-classification of immunoassays -- 2. History of ultrasensitive enzyme immunoassay -- 3. Factors limiting the sensitivity of noncompetitive heterogeneous solid phase enzyme immunoasssays -- 4. Methods to minimize effects of factors limiting the sensitivity -- 5. Principle of the immune complex transfer enzyme immunoassay -- 6. Potential of the immune complex transfer enzyme immunoassay to improve the sensitivity and its limitations -- 7. Ultrasensitive and rapid enzyme immunoassay -- 8. Protocol of immune complex transfer enzyme immunoassay -- 9. Ultrasensitive immunoassay for haptens -- 10. Properties of the reagents for enzyme-labeling -- 11. Enzyme-labeling of antibodies -- 12. Preparation and affinity-purification of IgG fragments -- 13. Protocol for enzyme-labeling of antibodies -- 14. Protocol for labeling avidin with 2,4-dinitrophenyl groups and b-D-galactosidase -- 15. Enzyme-labeling of antigens -- 16. Protocol for enzyme-labeling of antigens -- 17. Assays of enzymes.
- ISBN
- 0444502017
- 9780444502018
- 0444502025
- 9780444502025
- 0720442001
- 9780720442007
- LCCN
- 99010292
- OCLC
- ocm40632809
- 40632809
- SCSB-929390
- Owning Institutions
- Princeton University Library