Research Catalog

Title

The nucleic acid protocols handbook / edited by Ralph Rapley.

Publication

Totowa, N.J. : Humana Press, ©2000.

Items in the Library & Off-site

Details

Additional Authors

Rapley, Ralph.

Description

xxii, 1050 pages : illustrations; 26 cm

Subject

• Nucleic acids > Laboratory manuals
• Polymerase chain reaction > Laboratory manuals
• Gene mapping > Laboratory manuals
• Molecular genetics > Laboratory manuals
• Nucleic Acids > isolation & purification
• Chromosome Mapping > methods
• Molecular Biology > methods
• Polymerase Chain Reaction > methods
• Chromosomes
• Biologie moléculaire
• Manuels
• ADN = Acide désoxyribonucléique
• Gene mapping
• Molecular genetics
• Nucleic acids
• Polymerase chain reaction
• Acides nucléiques
• Réaction de polymérisation en chaîne
• Cartographie chromosomique
• Nucleinsäuren
• Aufsatzsammlung
• Acides nucléiques > Manuels de laboratoire
• PCR (génétique) > Manuels de laboratoire
• Liaison génétique > Manuels de laboratoire
• Génétique moléculaire > Manuels de laboratoire
• Chromosomes > Cartes > Manuels de laboratoire

Genre/Form

• Laboratory Manual.
• Laboratory manuals.
• Manuels de laboratoire.

Bibliography (note)

• Includes bibliographical references and index.

Contents

• Pt. I. Nucleic Acid Extraction -- 1. Isolation of High-Molecular-Weight DNA from animal Cells / Ian Garner -- 2. Isolation of mRNA by Affinity Chromatography / Sian Bryant and David L. Manning -- 3. Isolation and Purification of DNA from Plants / Justin Stacey and Peter G. Isaac -- 4. Purification of Uncontaminated, Intact Plant RNA / Shu-Hua Cheng, Brandon D. Moore and Jeffrey R. Seemann -- 5. Improved Method to Isolate Mitochondrial RNA from Green Plant Tissue / Fei Ye and Ralf Reski -- 6. Isolating Chromosomal DNA from Bacteria / Elisabeth Chachaty and Patrick Saulnier -- 7. Bacterial DNA Extraction for Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis / Elisabeth Chachaty and Patrick Saulnier -- 8. Isolation of Fungal Nucleic Acids / Surapareddy Sreenivasaprasad -- 9. Total RNA Isolation from Bacteria / John Heptinstall -- 10. Simultaneous RNA and DNA Extraction from Biopsy Material, Culture Cells, Plants, and Bacteria / Udo Dobbeling -- 11. Spectrophotometric Analysis of Nucleic Acids / John Heptinstall and Ralph Rapley -- Pt. II. Basic Separation and Analysis of DNA -- 12. Restriction Endonuclease Digestion of DNA / Duncan R. Smith -- 13. Agarose Gel Electrophoresis of Nucleic Acids / D. Ross Williams and Ralph Rapley -- 14. Preparation of RNA Dot Blots / Rachel Hodge -- 15. Native Polyacrylamide Gel Electrophoresis / Adrian J. Harwood -- 16. Southern Blotting of Agarose Gels by Capillary Transfer / Ralph Rapley and Jane Davenport-Jones -- 17. Pulsed-Field Gel Electrophoresis / John Maule -- 18. HPLC of DNA and PCR Products / Elena D. Katz -- Pt. III. Probe Design, Synthesis, and Labeling -- 19. End-Labeling of DNA Fragments / Adrian J. Harwood -- 20. Nick Translation and Random Hexamer Labeling of DNA / Jane Davenport-Jones -- 21. Generation of Labeled Probes by Polymerase Chain Reaction / Y. M. Dennis Lo and Shu F. An
• 22. Nonradioactive Oligonucleotide Probe Labeling / Sue Fowler and Ian Durrant -- 23. Preparation of Direct, Enzyme-Labeled DNA Probes / Ian Durrant and Timothy Stone -- 24. Random Prime Labeling of DNA Probes with Fluorescein-Tagged Nucleotides / Bronwen M. Harvey, Claire B. Wheeler and Martin W. Cunningham -- 25. Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Chemiluminescence / Claire B. Wheeler, Bronwen M. Harvey and Martin W. Cunningham -- 26. Hybridization of Enzyme-Labeled Probes and Detection by Chemiluminescence / Timothy Stone and Ian Durrant -- 27. Hybridization and Competition Hybridization of Southern Blots / Rosemary Kelsell -- 28. Autoradiography and Fluorography / Eric Quemeneur -- Pt. IV. RNA Analysis Techniques -- 29. Formaldehyde Gel Electrophoresis of Total RNA / Sian Bryant and David L. Manning -- 30. RNA Probes for the Analysis of Gene Expression / Dominique Belin -- 31. Primer Extension Analysis of mRNA / Maggie Walmsley, Mark Leonard and Roger Patient -- 32. S1 Mapping Using Single-Stranded DNA Probes / Stephane Viville and Roberto Mantovani -- 33. Measurements of Rate of Transcription in Isolated Nuclei by Nuclear "Run-Off" Assay / Rai Ajit K. Srivastava and Gustav Schonfeld -- 34. One-Tube RT-PCR with Sequence-Specific RT Primers / Ulrich Pfeffer and Paola Ferro -- 35. Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA / Francois Mallet -- 36. Quantitative Analysis of RNA Species by Polymerase Chain Reaction and Solid-Phase Minisequencing / Anu Suomalainen and Ann-Christine Syvanen -- 37. Nonradioactive Northern Blotting of RNA / Rainer Low -- 38. Analysis of RNA by Northern blotting Using Riboprobes / Rai Ajit K. Srivastava -- Pt. V. Gene Library Construction and Screening -- 39. Production of Double-Stranded cDNA for Gene Library Synthesis / Jane Kirk and Steve Mayall
• 40. Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs / Yue Zhang and Michael A. Frohman -- 41. cDNA Library Construction Using Streptavidin-Paramagnetic Beads and PCR / Kris N. Lambert and Valerie M. Williamson -- 42. Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products / Alan R. Shuldiner and Keith Tanner -- 43. Subtraction Hybridization cDNA Libraries / Clifford W. Schweinfest, Peter S. Nelson and Michael W. Graber / [et al.] -- 44. Cloning Polymerase Chain Reaction Products Utilizing the T/A Overhang and a Kit / Melissa Lail-Trecker -- 45. Extraction and Purification of Plasmid DNA / Craig Winstanley and Ralph Rapley -- 46. Biotinylated Probes in Colony Hybridization / Michael J. Haas -- 47. Cloning Long Polymerase Chain Reaction Products / Songrong Ren and J. Michael Ruppert -- 48. Cloning DNA Fragments in M13 Vectors / David Walsh -- 49. cDNA Library Construction for the Lambda ZAP-Based Vectors / Marjory A. Snead, Michelle A. Alting-Mees and Jay M. Short -- 50. Expression and Preparation of Fusion Proteins from Recombinant [lambda]gt11 Phages / Sheng-He Huang and Ambrose Jong -- 51. Antibody Screening of Bacteriophage [lambda]gt11 DNA Expression Libraries / Peter Jones -- 52. Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides / Caroline A. Austin -- 53. cDNA Library Screening with the Tetramethylammonium Chloride (TMAC) Technique Using Highly Degenerate Oligoonucleotide Probes / Bent Honore and Peder Madsen -- 54. Screening Recombinant Libraries by Polymerase Chain Reaction / Michael W. King -- 55. Construction and Screening of Cosmid Libraries / Jens Hanke and Jorg D. Hoheisel -- 56. Generation of Large Insert YAC Libraries / Zoia Larin, Anthony P. Monaco and Hans Lehrach -- 57. YAC Library Storage and Transport / John E. Collins, Sheila Hassock and Ian Dunham
• 58. YAC Library Screening: Preparation of Hybridization Filters and Polymerase Chain Reaction Pools / Charlotte G. Cole, John E. Collins and Ian Dunham -- 59. YAC Library Screening: Hybridization and PCR-Based Screening Protocols / Charlotte G. Cole, John E. Collins and Ian Dunham -- 60. Phage-Display Libraries of Murine and Human Antibody Fab Fragments / Jan Engberg, Lene K. Johansen and Michelle Westengaard-Hildinge / [et al.] -- Pt. VI. DNA Sequencing -- 61. Preparation and Analysis of DNA Sequencing Gels / Bimal D. M. Theophilus -- 62. DNA Sequencing of Plasmids / George Murphy -- 63. Sequencing DNA Fragments Cloned into M13 and Phagemid Vectors / Neil Brewis -- 64. Direct cDNA Sequencing Using Sequential Linear/Asymmetric Polymerase Chain Reaction / Ivor J. Mason -- 65. Purification and Enzymatic Sequencing of Polymerase Chain Reaction Products / Frank C. Brosius III, Lawrence B. Holzman and Xinan Cao -- 66. Direct Polymerase Chain Reaction Sequencing with Denaturants / Wei Zhang and Albert B. Deisseroth -- 67. Direct DNA Sequencing of Polymerase Chain Reaction Products Using Magnetic Beads / Joakim Lundeberg, Bertil Pettersson and Mathias Uhlen -- 68. Polymerase Chain Reaction Cycle Sequencing with Degenerate Primers / Zhiyuan Shen, Jingmei Liu and Robert L. Wells / [et al.] -- 69. Direct Automated Cycle Sequencing of Polymerase Chain Reaction Products / Susan E. Daniels -- 70. Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products / Anu Suomalainen and Ann-Christine Syvanen -- 71. DNA Sequencing by the Chemical Method / Eran Pichersky -- 72. One-Step One-Lane Chemical Sequencing of DNA / Giovanna Costanzo, Ernesto Di Mauro and Rodolfo Negri -- Pt. VII. Basic Polymerase Chain Reaction Methods -- 73. Polymerase Chain Reaction: Basic Principles and Routine Practice / Lori A. Kolmodin and J. Fenton Williams
• 74. Primer Selection and Design for Polymerase Chain Reaction / Wojciech Rychlik -- 75. One-Step Optimization Using Touchdown and Stepdown Polymerase Chain Reaction / Kenneth H. Roux and Karl H. Hecker -- 76. Cloning Gene Family Members Using Polymerase Chain Reaction with Degenerate Oligonucleotide Primers / Gregory M. Preston -- 77. Construction of Synthetic Genes by Polymerase Chain Reaction / Patrick J. Dillon and Craig A. Rosen -- 78. Rapid Amplification of cDNA Ends / David Bertioli -- 79. Multiplex Polymerase Chain Reaction / Jerald Radich -- 80. Inverse Polymerase Chain Reaction / Sheng-He Huang -- 81. Long Range Polymerase Chain Reaction / William Waggott -- Pt. VIII. Analyzing Genes, Mutations, and Protein Interactions -- 82. Nonradioactive Differential Display of Messenger RNA / Thomas C. G. Bosch and Jan U. Lohmann -- 83. Gene Isolation by Exon Trapping / David B. Krizman -- 84. DNA Rescue by the Vectorette Method / Marcia A. McAleer, Alison Coffey and Ian Dunham -- 85. Random Amplified Polymorphic DNA (RAPDs) / Scott Tingey -- 86. Restriction Fragment Length Polymorphism / Mohammad S. Enayat -- 87. Detection of Mutations in DNA and RNA by Chemical Cleavage / Richard G. H. Cotton -- 88. Mutation Screening Using PCR-SSCP: Silver Staining and Isotopic Protocols / Philip J. Saker -- 89. Detecting Point Mutations by Denaturing-Gradient Gel Electrophoresis / Stephen R. Dlouhy, Patricia Wheeler and James A. Trofatter / [et al.] -- 90. Analysis of Nucleotide Sequence Variation by Solid-Phase Minisequencing / Anu Suomalainen and Ann-Christine Syvanen -- 91. Amplification Refractory Mutation System / John M. Old -- 92. DNase I Footprinting / Benoit Leblanc and Tom Moss -- 93. Identification of Protein-DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference / Peter E. Shaw and A. Francis Stewart
• 94. Gel Shift Assay for the Analysis of DNA-Protein Interactions / John D. Taylor, Alison J. Ackroyd and Stephen E. Halford -- 95. Yeast Two-Hybrid Library Screening / Ian G. Cowell -- 96. Southwestern Assay / Jacques Philippe -- 97. Nonradioactive Methods for the Detection of RNA-Protien Interaction / Asier Echarri, Maria Eugenia Gonzalez and Ivan Ventoso / [et al.] -- 98. Transcriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay / David R. Hodge, Delores M. Thompson and Alexandra Panayiotakis / [et al.] -- Pt. IX. Mutagenesis, Transcription, and Translation In Vitro -- 99. Generating Nested Deletions with Exonuclease III / George Murphy -- 100. Primer-Directed Site-Specific Mutagenesis / Michael J. O'Donohue and G. Geoff Kneale -- 101. Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template / Christian Hagemeier -- 102. Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates / Jeffrey Bramam, Carol Papworth and Alan Greener -- 103. Site-Directed Mutagenesis with LA-PCR Technology / Atsushi Shimada and Osamu Takeda -- 104. Recombination and Mutagenesis by Overlap Extension PCR / Robert J. Pogulis, Abbe N. Vallejo and Larry R. Pease -- 105. Site-Directed Mutagenesis and Gene Fusion by Megaprimer PCR / Sailen Barik -- 106. Transcription In Vitro Using Bacteriophage RNA Polymerases / Elaine T. Schenborn -- 107. In Vitro Translation of mRNA in a Rabbit Reticuloctye Lysate Cell-Free System / Louise Olliver and Charles D. Boyd -- 108. In Vitro Translation of mRNA in a Wheat Germ Extract Cell-Free System / Louise Olliver, Anne Grobler-Rabie and Charles D. Boyd -- 109. Xenopus Egg Extract Translation System / Glenn M. Matthews and Alan Colman -- 110. Manipulation of Baculovirus Vectors / Claire L. Merrington, Mark J. Bailey and Robert D. Possee
• 111. Procedures for the Analysis and Purification of His-Tagged Proteins / Richard E. Kneusel, Joanne Crowe and Melanie Wulbeck / [et al.] -- 112. Detection and Immobilization of Proteins Containing the 6xHis Tag / Richard E. Kneusel, Melanie Wulbeck and Joachim Ribbe -- 113. Expression and Purification of Recombinant Proteins Using the [rho]ET System / Robert C. Mierendorf, Barbara B. Morris and Beth Hammer -- Pt. X. Gene Localization, Mapping In Situ, and Bioinformatics -- 114. Preparation of Tissue Sections and Slides for mRNA Hybridization / Giorgio Terenghi -- 115. Use of Digoxigenin-Labeled Probes on Tissue Sections / Giorgio Terenghi -- 116. Gene Mapping by FISH / Rafael Espinosa III and Michelle M. Le Beau -- 117. Oligonucleotide PRINS DNA Synthesis / John R. Gosden and Diane Lawson -- 118. Chromosome-Specific PRINS / Jean-Paul Charlieu and Frank Pellestor -- 119. In Situ PCR Amplification of Intracellular mRNA / Raymond H. Chen and Susan V. Fuggle -- 120. Introduction to Bioinformatics / Henry Brzeski.

ISBN

• 0896034593
• 9780896034594
• 0896038416
• 9780896038417

LCCN

98043385

OCLC

• ocm39868199
• 39868199
• SCSB-14408734

Owning Institutions

Princeton University Library