Research Catalog

Title

An introduction to immunocytochemistry : current techniques and problems / Julia M. Polak and Susan Van Noorden.

Author

Polak, Julia M.

Publication

Oxford ; New York : Oxford University Press ; Oxford : Royal Microscopical Society, 1987.

Items in the Library & Off-site

Details

Additional Authors

• Van Noorden, Susan.
• Royal Microscopical Society (Great Britain)

Description

60 pages : illustrations (some color); 24 cm.

Series Statement

• Microscopy handbooks ; 11
• Oxford science publications

Uniform Title

• Microscopy handbooks ; 11.
• Oxford science publications.

Subject

• Immunocytochemistry
• Immunochemistry
• Histocytochemistry
• Immunochemistry
• Immunohistochemistry
• Immunocytochemistry
• Immunohistochimie
• Microscopie
• Imunologia Medica
• Imunologia
• Imunologia (Tecnicas)

Note

• Includes index.

Bibliography (note)

• Bibliography: p. [52]-58.

Contents

1. Introduction -- 2. Production of Antibodies -- 3. Requirements for Immunocytochemistry -- 4. Immunofluorescence Methods -- 5. Immunoenzyme methods. -- 6. Modifications of the Basic Methods -- 7. Specificity Problems and Essential Controls -- 8. In Vitro Methods for Testing Antibodies and Checking Antigens -- 9. Applications of Immunocytochemistry -- 10. Microscopy -- 11. Appendix: Technical Notes -- A.1. Immunostaining by Indirect Method -- A.2. Peroxidase Antiperoxidase Method -- A.3. Cryostat Sections -- A.4. Intensification of the Peroxidase Reaction -- A.5. Protease Digestion -- A.6. To Prevent Sections Becoming Detached from Slides -- A.7. Peroxidase Development by Alternatives to DAB Methods -- A.8. Notes -- A.9. Development of Alkaline Phosphabase to Give a Blue End-Product -- A.10. Post-Embedding Electron Microscopical Immunocytochemistry Using an Indirect Immunogold Method

ISBN

• 0198564155
• 9780198564157
• 9780198564119
• 0198564112
• 0198564112 (canceled/invalid)

LCCN

86023910

OCLC

• ocm14692192
• 14692192
• SCSB-1167649

Owning Institutions

Princeton University Library