Research Catalog

Title

RNA interference (RNAi) : nuts & bolts of RNAi technology / edited by David R. Engelke.

Publication

[Eagleville, PA] : DNA Press, ©2003.

Items in the Library & Off-site

Details

Additional Authors

Engelke, David R. (David Ross)

Description

viii, 236 pages : illustrations; 22 cm.

Summary

This work focuses on practical approaches to and uses of a revolutionary technology called RNAi for RNA interference. A recently developed research tool, this process allows scientists to knock out or knock down the levels of a gene product, shut off targeted genes in an organism, and then monitor the results within that organism. Leading academic and biotechnology researchers in various fields that utilize this process introduce and explain a specific model system and use of RNA interference as well as necessary background information. Tools for the Arabidopsis, Caenrhabditis, and Drosophila model organism systems are offered, as is an overview of siRNA design and RNA transfection processes in mammalian cells. A discussion of chemical and enzymatic synthesis and preparation of siRNA in vitro is also included.

Series Statement

Nuts & bolts series

Uniform Title

Nuts & bolts series

Alternative Title

Ribonucleic acid interference (RNAi)

Subject

• RNA
• Gene silencing
• Small interfering RNA
• RNA Interference > physiology
• RNA, Small Interfering
• Biotechnology > methods
• RNA
• Small interfering RNA
• Gene silencing

Bibliography (note)

• Includes bibliographical references and index.

Contents

Section I. RNA interference as an experimental tool in invertebrates. -- Chapter 1. Targeted gene silencing in plants using RNA interference -- Chapter 2. RNAi in caenorhabditis elegans -- Chapter 3. Application and analysis of RNAi in drosophila systems -- Section II. Short interfering RNA as a tool in vertebrate systems: synthesizing the RNA -- Chapter 4. Gene silencing by synthetic siRNA duplexes in mammalian cell culture -- Chapter 5. Strategies for synthesizing small interfering RNA (siRNA) -- Chapter 6. Generating long dsRNA, individual siRNA and siRNA cocktails in vitro -- Section III. Short interfering RNA as a tool in vertebrate systems: applications -- Chapter 7. Optimizing high throughput RNAi-based assays using transient transfection of synthetic siRNAs in cultured mammalian cells -- Chapter 8. Subcellular delivery and detection of small interfering RNA -- Chapter 9. Downregulation of cellular genes by PCR products expressing siRNAs mammalian cells -- Chapter 10. Generating adenoviruses for shRNA or siRNA delivery -- Chapter 11. Achieving stable, heritable gene silencing in the mouse.

ISBN

• 9780966402780
• 0966402782

OCLC

• ocm55113007
• 55113007
• SCSB-9621519

Owning Institutions

Princeton University Library