Research Catalog

Title

Mitochondria / edited by Liza A. Pon, Eric A. Schon.

Publication

Amsterdam ; Boston : Academic Press, ©2007.

Items in the Library & Off-site

Details

Additional Authors

• Pon, Liza A.
• Schon, Eric A.

Description

xxiv, 920 pages : illustrations.

Summary

This book provides an update on the step-by-step "how to" methods for the study mitochondrial structure, function, and biogenesis contained in the successful first edition. As in the previous edition, the biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls from each method.

Series Statement

Methods in cell biology, 0091-679X ; v. 80

Uniform Title

Methods in cell biology ; v. 80.

Subject

• Mitochondria
• Mitochondria
• mitochondria
• oxidation
• stress
• measurement
• gene expression
• analytical methods
• Labortechnik
• Mitochondrium
• Molekularbiologie
• Mitochondriën
• Mitochondries

Note

• Earlier edition published 2001.

Bibliography (note)

• Includes bibliographical references and index.

Contents

Isolation and subfractionation of mitochondria from animal cells and tissue culture lines / F. Pallotti and G. Lenaz -- Purification and subfractionation of mitochondria from the yeast Saccharomyces cerevisiae / I.R. Boldogh and L.A. Pon -- Isolation and subfractionation of mitochondria from plants / A.H. Millar, A. Liddell and C.J. Leaver -- Biochemical assays of respiratory chain complex activity / D.M. Kirby [and others] -- Polarographic assays of respiratory chain complex activity / G. Villani and G. Attardi -- Optical imaging techniques (histochemical, immunohistochemical, and in situ hybridization staining methods) to visualize mitochondria / K. Tanji and E. Bonilla -- Assay of mitochondrial ATP synthesis in animal cells and tissues / C. Vives-Bauza, L. Yang and G. Manfredi -- Measurement of the ratio of lactate to pyruvate in skin fibroblast cultures / N. Mackay and B.H. Robinson -- Assays of fatty acid beta-oxidation activity / M.J. Bennett -- Biochemical assays for mitochondrial activity: assays of TCA cycle enzymes and PDHc / A.S. Reisch and O. Elpeleg -- Assays of cardiolipin levels / M. Schlame -- Measurement of VDAC permeability in intact mitochondria and in reconstituted systems / M. Colombini -- Methods for studying iron metabolism in yeast mitochondria / S. Molik, R. Lill and U. Muhlenhoff -- Imaging of mitochondrial polarization and depolarization with cationic fluorophores / J.J. Lemasters and V.K. Ramshesh -- Biosensors for the detection of calcium and pH / P. Pinton [and others] -- Measurement of membrane permeability and the permeability transition of mitochondria / N. Zamzami [and others] -- Luciferase expression for ATP imaging: application to cardiac myocytes / C.J. Bell [and others] -- Measurement of reactive oxygen species in cells and mitochondria / J.S. Armstrong and M. Whiteman -- Measurements of the antioxidant enzyme activities of superoxide dismutase, catalase, and glutathione peroxidase / C. Vives-Bauza, A. Starkov and E. Garcia-Arumi -- Methods for measuring the regulation of respiration by nitric oxide / S. Shiva, P.S. Brookes and V.M. Darley-Usmar -- Methods for determining the modification of protein thiols by reactive lipids / J. Oh, M.S. Johnson and A. Landar -- Detection of mutations in mtDNA / A. Naini and S. Shanske -- Diagnostic assays for defects in mtDNA replication and transcription in yeast and humans / G.S. Shadel and B.L. Seidel-Rogol -- Microdissection and analytical PCR for the investigation of mtDNA lesions / S.L. Williams and C.T. Moraes -- Transmitochondrial technology in animal cells / S.R. Bacman and C.T. Moraes -- Genetic transformation of Saccharomyces cerevisiae and Chlamydomonas reinhardtii mitochondria / N. Bonnefoy, C. Remacle and T.D. Fox -- Generation of transmitochondrial mice: development of xenomitochondrial mice to model neurodegenerative diseases / C.A. Pinkert and I.A. Trounce -- In vivo and in organello analyses of mitochondrial translation / P. Fernandez-Silva [and others] -- Visualization of mitochondria in budding yeast / T.C. Swayne, A.C. Gay and L.A. Pon -- Visualization and quantification of mitochondrial dynamics in living animal cells / K.J. De Vos and M.P. Sheetz -- Cell-free assays for mitochondria-cytoskeleton interactions / I.R. Boldogh [and others] -- In vitro assays for mitochondrial fusion and division / E. Ingerman [and others] -- Electrophoretic methods to isolate protein complexes from mitochondria / I. Wittig and H. Schagger -- Analysis of protein-protein interactions in mitochondria / J.M. Herrmann and B. Westermann -- Analysis and prediction of mitochondrial targeting signals / S.J. Habib, W. Neupert and D. Rapaport -- Import of proteins into mitochondria / D. Stojanovski, N. Pfanner and N. Wiedemann -- Appendix 1. Basic properties of mitochondria / L.J. Garcia-Rodriguez -- Appendix 2. Direct and indirect inhibitors of mitochondrial ATP synthesis / N. Orme-Johnson -- Appendix 3. Linearized maps of circular mitochondrial genomes from representative organisms / E.A. Schon -- Appendix 4. Mitochondrial genetic codes in various organisms / E.A. Schon -- Appendix 5. Gene products present in mitochondria of yeast and animal cells / E.A. Schon -- Appendix 6. Changes in the mitochondrial transcriptome and proteome under various stresses and growth conditions / E.A. Schon.

ISBN

• 9780125441735
• 0125441738

OCLC

• ocn123081114
• 123081114
• SCSB-1428277

Owning Institutions

Princeton University Library