Research Catalog

Title

PCR : clinical diagnostics and research / A. Rolfs [and others].

Publication

Berlin ; New York : Springer-Verlag, ©1992.

Items in the Library & Off-site

Details

Additional Authors

Rolfs, Arndt, 1959-

Description

xix, 271, 96 pages : illustrations; 26 cm

Series Statement

Springer laboratory

Uniform Title

Springer laboratory

Subject

• Polymerase chain reaction
• Polymerase Chain Reaction > methods
• Polymerase Chain Reaction
• Réaction de polymérisation en chaîne
• Polymerase kettingreactie
• PCR (génétique)

Bibliography (note)

• Includes bibliographical references and index.

Contents

PCR: principles and reaction components -- Optimization strategies -- General applications of PCR -- Substances affecting PCR: inhibition or enhancement -- PCR: contamination and falsely interpreted results -- Biological material amenable to PCR -- Isolation of DNA from cells and tissue for PCR -- Isolation of RNA from cells and tissue for PCR -- Reverse transcription/PCR (RT-PCR) -- Methods for identification of amplified PCR products -- Restriction fragment analysis -- Multiplex PCR -- Detection of single base changes using PCR -- Non-radioactive, direct, solid-phase sequencing of genomic DNA obtained from PCR -- Application of PCR to analyze unknown sequences -- Quantification of PCR-products -- Cloning methods using PCR -- Site-directed mutagenesis using the PCR -- In-situ polymerase chain reaction -- Oligonucleotides in the field of PCR -- Review of different heat-stable DNA polymerases -- Physical features of thermocyclers and of their influence on the efficiency of PCR amplification -- Alternative methods to PCR.

ISBN

• 3540554408
• 9783540554400
• 0387554408
• 9780387554402

LCCN

92049560

OCLC

• ocm26636611
• 26636611
• SCSB-14400196

Owning Institutions

Princeton University Library