Research Catalog

Title

Monoclonal antibodies / J.H. Peters, H. Baumgarten (eds.) ; with contributions by numerous authors ; foreword by Georges Köhler ; [translated by Paul Debbage].

Publication

Berlin ; New York : Springer-Verlag, ©1992.

Items in the Library & Off-site

Details

Additional Authors

• Peters, J. H. (Johann Hinrich)
• Baumgarten, H. (Horst)

Description

xvii, 488 pages : illustrations; 25 cm

Series Statement

Springer laboratory

Uniform Title

• Monoklonale Antikörper. English.
• Springer laboratory.

Subject

• Monoclonal antibodies > Laboratory manuals
• Monoclonal antibodies
• Antibodies, Monoclonal
• Anticorps monoclonal > Manuel de laboratoire
• Monoklonale antistoffen
• Clonage moléculaire
• Anticorps monoclonaux
• Hybridomes

Genre/Form

• Laboratory Manual
• Laboratory manuals.
• Manuels de laboratoire.

Bibliography (note)

• Includes bibliographical references and index.

Contents

• 1. Introduction -- 1.1. Principles of Cell Hybridization -- 1.2. Properties and Significance of Monoclonal Antibodies -- 1.3. Use of Monoclonal Antibodies in Human Beings: Quality Control and Legal Aspects -- 2. Preconditions for Hybridoma Technology -- 2.1. Experimental Work with Animals -- 2.2. Equipment of the Cell Culture Laboratory -- 2.3. Equipment for Immunological and Biochemical Work -- 2.4. Organization of the Course of Work (Time Table) and Estimation of Costs -- 3. Immunization -- 3.1. Principles and Strategies for Immunizing Animals -- 3.2. Choice of the Immunogen -- 3.3. Immunizing the Larger Experimental Animals for Antisera Production -- 3.4. Immunizing Mice -- 3.5. Influencing the Immune Response -- 4. Taking Blood and Isolating Cells -- 4.1. Taking Blood from Experimental Animals -- 4.2. Isolating Lymphocytes from Spleen and Lymph Nodes -- 4.3. Isolating Human Lymphocytes from Peripheral Blood, Tonsils, or Spleen -- 4.4. Enriching Antigen-Specific Lymphoblasts for Fusion -- 4.5. Isolating Mouse Peritoneal Macrophages for Use as Feeder Cells -- 5. Cell Culture -- 5.1. Requirements for Cell Culture -- 5.2. Additives to Media: Growth Factors, Conditioned Media -- 5.3. Cryopreservation of Cells -- 5.4. Bacterial and Fungal Infections -- 5.5. Limiting an Infection in Multi-Well Plates -- 5.6. Mycoplasmas -- 5.7. Cell Viability Testing Using Fluorescent Dyes -- 6. Production of Hybridomas -- 6.1. Basics
• 6.2. Fusing Cells to Generate Mouse Monoclonal Antibodies -- 6.3. Human Hybridoma Technique -- 6.4. Other Fusion Methods -- 6.5. Calculating the Number of Hybridoma Clones To Be Expected -- 6.6. Culture and Enrichment of Hybridomas -- 6.7. Cloning Cells -- 6.8. Identifying Human Genomic Material in Mouse-Human Hybridomas -- 6.9. Fine-Tuning Hybridomas -- 6.10. Nomenclature of Monoclonal Antibodies -- 7. Mass Production of Monoclonal Antibodies -- 7.1. Mass Production of Monoclonal Antibodies in Cell Culture or Ascites -- 7.2. Production of Monoclonal Antibodies in Mice -- 7.3. Production of Monoclonal Antibodies in Bioreactors -- 7.4. Serum-Free Cell Culture -- 7.5. Checking the Antibody Properties -- 8. Purifying Monoclonal Antibodies and Producing Antibody Fragments -- 8.1. Purification of Monoclonal Antibodies: an Overview -- 8.2. Producing Immunoreactive Fragments from Mouse Monoclonal Antibodies -- 9. Coupling Monoclonal Antibodies -- 9.1. Basic Principles -- 9.2. Conjugation of Enzymes to Monoclonal Antibodies -- 9.3. Biotinylating Monoclonal Antibodies -- 9.4. Conjugating Fluorochromes to Monoclonal Antibodies -- 9.5. Conjugating Monoclonal Antibodies to Solid Phases (Immune Absorption) -- 10. Demonstration of Monoclonal Antibodies -- 10.1. How To Find the Correct Monoclonal Antibody -- 10.2. Immunoassays for Soluble Antigens: a Survey -- 10.3. ELISA for Demonstration of Monoclonal Antibodies Against Soluble Antigens
• 10.4. Quantitative Tests to Demonstrate the Synthetic Capacity of Hybridoma Cells -- 10.5. Selection of a Test System for Antibodies Against Cellular Antigens -- 10.6. Immunofluorescence Demonstration of Cytoplasmic Ig in Fixed Lymphocytes -- 10.7. Immunofluorescence Demonstration of Membrane Antigens on Living Lymphocytes -- 10.8. Immunocytochemical Staining Techniques -- 10.9. Immunocytochemical Demonstration of Membrane Antigens on Living Cells -- 10.10. ELISA Demonstration of Antigens in Fixed Cells (Cell ELISA) -- 10.11. Local Demonstration of Specific Antibody -- 10.12. Dot Immunobinding Test -- 10.13. Molecular Weight Determination of Membrane Antigens by Means of Chemiluminescence-Autography and Sequential Immunoprecipitation -- 10.14. Depletion of Cells in Suspension by Use of Particle-Bound Antibodies (Magnetic Particles) -- 10.15. Typing Class and Subclass (Isotyping) of Mouse Antibodies by Means of ELISA -- 10.16. Analytical HPLC of Monoclonal Antibodies -- 10.17. Analytical SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) -- 10.18. Analytical Isoelectric Focusing of Monoclonal Antibodies -- 10.19. Silver Staining of Polyacrylamide Gels -- 10.20. Protein Blotting, Immunoblotting ("Western Blot") -- 10.21. Epitope Analysis -- 11. Safety Precautions at Work -- 12. Appendix -- 12.1. Monographs -- 12.2. Reference Works for Obtaining Cells, Reagents, and Laboratory Equipment -- 12.3. Addresses of Firms.

ISBN

• 3540508430
• 9783540508434
• 0387508430
• 9780387508436
• 3387508430 (canceled/invalid)

LCCN

92030163

OCLC

• ocm26400572
• 26400572
• SCSB-2022965

Owning Institutions

Princeton University Library