Research Catalog
Molecular genetics of yeast : a practical approach
- Title
- Molecular genetics of yeast : a practical approach / edited by John R. Johnston.
- Publication
- Oxford ; New York : IRL Press at Oxford University Press, ©1994.
Items in the Library & Off-site
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1 Item
| Status | Format | Access | Call Number | Item Location |
|---|---|---|---|---|
| Text | Use in library | QH470.S23 M65 1994 | Off-site |
Details
- Additional Authors
- Johnston, John R.
- Description
- xxiv, 275 pages : illustrations; 24 cm
- Summary
- The yeast Saccharomyces cerevisiae is used extensively in academic research as a model eukaryote, and is also one of the most important industrial organisms. It is easy to manipulate, and has a great many parallels with higher organisms, making it ideal for studies in molecular biology, genetics, and biotechnology. This book describes current experimental procedures, written by internationally recognized experts, for working with this organism. Topics covered include DNA isolation, cloning and expression vectors, construction and use of DNA libraries, Ty insertional mutagenesis, high-efficiency transformation, cell-free translation of mRNAs, virus-like particles, and aspects of industrial strains. Molecular Genetics of Yeast: A Practical Approach provides a comprehensive compendium of detailed, clearly presented protocols, with additional practical tips, for all researchers working with this organism.
- Series Statement
- The practical approach series ; 141
- Uniform Title
- Practical approach series ; 141.
- Subject
- Saccharomyces cerevisiae > Genetics
- Molecular genetics > Technique
- Genetics, Experimental
- Genetics > Technique
- Saccharomyces cerevisiae > genetics
- Genetic Techniques
- Molecular Biology
- Yeasts > genetics
- Genetics > Technique
- Genetics, Experimental
- Molecular genetics > Technique
- Saccharomyces cerevisiae > Genetics
- Saccharomyces cerevisiae > Génétique
- Biologie moléculaire
- Levures > Génétique
- Hefeartige Pilze
- Methode
- Molekulargenetik
- Saccharomyces cerevisiae
- Champignons > Génétique
- Génétique moléculaire
- Saccharomyces cerevisiae
- Bibliography (note)
- Includes bibliographical references and index.
- Contents
- Procedures for isolating yeast DNA for different purposes / Achim Wach, Horst Pick, Peter Philippsen -- Construction of cloning and expression vectors / Christopher Hadfield -- Cosmid cloning of yeast DNA / Rolf Stucka, Horst Feldmann -- Construction and use of cDNA libraries for genetic selections / John T. Mulligan, Stephen J. Elledge -- Pulsed field gel electrophoresis / John R. Johnston -- Plasmid shuffling and mutant isolation / Robert S. Sikorski, Jill B. Keeney, Jef D. Boeke -- Ty insertional mutagenesis / J.N. Strathern [and others].
- High efficiency transformation with lithium acetate / R. Daniel Gietz, Robin A. Woods -- Measurement of transcription / Peter W. Piper -- Measurement of mRNA stability / Alistair J.P. Brown -- Production of foreign proteins at high level / Gerard Loison -- Cell-free translation of natural and synthetic mRNAs in yeast lysates / Mick F. Tuite, Delin Zhu, Alan D. Hartley -- Virus-like particles: ty retrotransposons / Alan J. Kingsman [and others] -- The iller toxin: molecular and genetic applications to secretion and cell surface assembly / Jeffrey L. Brown [and others].
- Immuno-electron microscopy / Michael W. Clark -- Industrial Saccharomyces yeasts / Morten C. Kielland-Brandt -- More common media and buffers -- Addresses of suppliers.
- ISBN
- 0199634300
- 0199634297
- 9780199634309
- 9780199634293
- LCCN
- 93033833
- OCLC
- ocm29220888
- 29220888
- SCSB-9625186
- Owning Institutions
- Princeton University Library