Research Catalog

Handbook of molecular and cellular methods in biology and medicine

Title
Handbook of molecular and cellular methods in biology and medicine / Peter B. Kaufman [and others].
Publication
Boca Raton : CRC Press, ©1995.

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Details

Additional Authors
Kaufman, Peter B.
Description
484 pages : illustrations; 26 cm
Summary
Covers a range of current methods including some extensively tested techniques. Methods include: isolation and purification of DNA, RNA and proteins; preparation of hybridization probes; blotting techniques; construction of cDNA and genomic DNA libraries; and DNA sequencing.
Alternative Title
Molecular and cellular methods in biology and medicine
Subject
  • Molecular biology > Laboratory manuals
  • Cytology > Laboratory manuals
  • Cytology > Technique
  • Molecular Biology
  • Cytological Techniques
  • Cytology > Technique
  • Cytology
  • Molecular biology
  • Cytologie
  • Labortechnik
  • Methode
  • Molekularbiologie
  • Laboratoriumonderzoek
  • Cellen (biologie)
  • Moleculaire biologie
Genre/Form
  • Laboratory Manual
  • Laboratory manuals
  • Laboratory manuals.
  • Manuels de laboratoire.
Bibliography (note)
  • Includes bibliographical references and index.
Contents
  • Isolation and purification of DNA -- Isolation and purification of RNA -- Extraction and purification of proteins/enzymes -- Preparation of nucleic acid probes -- Electrophoresis, blotting and hybridization -- cDNA libraries -- Genomic DNA libraries -- DNA sequencing -- PCR techniques and applications -- DNA fingerprinting -- DNA mutagenesis in vitro -- Inhibition of gene expression by antisense DNA and RNA -- DNA footprinting and gel retardation assay -- Translation of mRNA(s) and analysis of proteins by gel electrophoresis.
  • Gene transfer and expression in animals -- Gene transfer and expression in plants -- Plant cell culture -- Plant tissue culture -- Microscopy: LM, SEM, TEM, confocal and applications -- Bioseparation techniques and their applications -- Preparation of monoclonal and polyclonal antibodies against specific protein(s).
ISBN
  • 0849325110
  • 9780849325113
LCCN
94022515
OCLC
  • ocm30809263
  • 30809263
  • SCSB-2053559
Owning Institutions
Princeton University Library